IL-27/IL-35 EBI3 Subunit Antibody

Images

 
Western Blot: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-03944] - Analysis of EBI3 in RAW cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 5 ug/ml. Goat anti-rabbit Ig HRP secondary ...read more
Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-03944] - Formalin-fixed, paraffin-embedded mouse brain tissue stained with EBI3 antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-03944] - Analysis of EBI3 in formalin-fixed, paraffin-embedded mouse brain tissue using an isotype control (top left) and (bottom left, right) at 5 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IL-27/IL-35 EBI3 Subunit Antibody Summary

Immunogen
A portion of amino acids 200-230 of mouse EBI3 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EBI3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in mouse (isoforms CRA_a and CRA_b) and rat and 72% homologous in human, dog and rhesus monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IL-27/IL-35 EBI3 Subunit Antibody

  • cytokine receptor
  • EBI3
  • EBV-induced gene 3 protein
  • Epstein-Barr virus induced 3
  • Epstein-Barr virus induced gene 3
  • Epstein-Barr virus-induced gene 3 protein
  • IL-27 subunit beta
  • IL-27/IL-35 EBI3 Subunit
  • IL27B
  • IL-27B
  • IL35 EBI3
  • interleukin-27 subunit beta

Background

EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively (Honglian Tong et al, 2010). IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis (Kerstin A et al, 2008).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EBI3
Entrez
Uniprot