IL-27/IL-35 EBI3 Subunit Antibody [HRP]

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Western Blot: IL-27/IL-35 EBI3 Subunit Antibody [HRP] [NBP1-42766] - Mouse EBI-3 Antibody Peroxidase Conjugated - Western Blot

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42766
    • Availability
      Product Discontinued

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IL-27/IL-35 EBI3 Subunit Antibody [HRP] Summary

Immunogen
This antibody was prepared by repeated immunizations with recombinant mouse EBI3.
Specificity
Cross reactivity does NOT occur with human EBI-3. Cross-reactivity with IL-27 from other sources has not been determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EBI3
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:20000-1:100000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. Expect a band approximately 34 kDa in size corresponding - the mature mouse EBI3 protein by western blotting in appropriate cell lysate or extract.
Readout System

Reactivity Notes

Mouse, rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
IgG purified
Reconstitution Instructions
Reconstitute with 50 ul of distilled water to a final concentration of 1.0 mg/ml.

Alternate Names for IL-27/IL-35 EBI3 Subunit Antibody [HRP]

  • cytokine receptor
  • EBI3
  • EBV-induced gene 3 protein
  • Epstein-Barr virus induced 3
  • Epstein-Barr virus induced gene 3
  • Epstein-Barr virus-induced gene 3 protein
  • IL-27 subunit beta
  • IL-27/IL-35 EBI3 Subunit
  • IL27B
  • IL-27B
  • IL35 EBI3
  • interleukin-27 subunit beta

Background

The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses.

The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EBI3
Entrez