IL-27/IL-35 EBI3 Subunit Antibody

Images

 
Western Blot: IL-27/IL-35 EBI3 Subunit Antibody [NBP1-42764] - Loaded on to an SDS-PAGE gel at 0.1 ug and after separation, transferred to nitrocellulose. The expected band is a non-glycosylated polypeptide chain ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Concentration
LYOPH mg/ml

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

IL-27/IL-35 EBI3 Subunit Antibody Summary

Immunogen
This antibody was prepared by repeated immunizations with recombinant mouse EBI3.
Specificity
Cross reactivity does NOT occur with human EBI-3. Cross-reactivity with IL-27 from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Gene
EBI3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
This product is and PBS and 2% Sucrose.
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Purity
IgG purified
Reconstitution Instructions
Reconstitute with 50 ul of distilled water to a final concentration of 1.0 mg/ml.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:1000-1:5000
Application Notes
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting.Expect a band approximately 23 kDa in size corresponding - the mature mouse EBI3 protein, a non-glycosylated polypeptide chain consisting of 207 amino acids, by western blotting in appropriate cell lysate or extract.

Reactivity Notes

Mouse, rat

Alternate Names for IL-27/IL-35 EBI3 Subunit Antibody

  • cytokine receptor
  • EBI3
  • EBV-induced gene 3 protein
  • Epstein-Barr virus induced 3
  • Epstein-Barr virus induced gene 3
  • Epstein-Barr virus-induced gene 3 protein
  • IL-27 subunit beta
  • IL-27/IL-35 EBI3 Subunit
  • IL27B
  • IL-27B
  • IL35 EBI3
  • interleukin-27 subunit beta

Background

The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses.

The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB15041
Species: Hu
Applications: WB, IHC, ICFlow
NBP2-35172
Species: Mu
H00009446-B01P
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP2-13440
Species: Hu
Applications: WB, IHC, IHC-P
NB100-1834
Species: Hu
Applications: WB, ICC/IF, Flow-IC
H00009527-B01P
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-84222
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-89530
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
BAF2428
Species: Hu
Applications: WB
NBP1-92564
Species: Hu
Applications: WB, IHC, IHC-P
MAB7918
Species: Hu
Applications: WB, Simple Western
AF7250
Species: Hu
Applications: IHC
NBP2-23485
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, IHC, IHC-P
NBP1-47842
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-42764
Species: Mu, Rt
Applications: WB, ELISA

Publications for IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764) (0)

There are no publications for IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764) (0)

There are no reviews for IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IL-27/IL-35 EBI3 Subunit Antibody Products

Related Products by Gene

Bioinformatics Tool for IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764)

Discover related pathways, diseases and genes to IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764)

Discover more about diseases related to IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764).
 

Pathways for IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764)

View related products by pathway.

PTMs for IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764)

Learn more about PTMs related to IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764).
 

Research Areas for IL-27/IL-35 EBI3 Subunit Antibody (NBP1-42764)

Find related products by research area.

Blogs on IL-27/IL-35 EBI3 Subunit

There are no specific blogs for IL-27/IL-35 EBI3 Subunit, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol EBI3
Entrez
Uniprot

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-42764 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought