IL-27/IL-35 EBI3 Subunit Antibody (15k8D10) - Azide Free

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Western Blot: IL-27/IL-35 EBI3 Subunit Antibody (15k8D10) - Azide Free [NBP2-27363] - analysis of Interleukin-35-Fc protein (A) and human Fc (B) using Interleukin-35 monoclonal antibody at 4 ug/ml.
Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody (15k8D10) - Azide Free [NBP2-27363] - Formalin-fixed, paraffin-embedded human spleen (left) and lymph node metastatic malignant melanoma (right) stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P
Clone
15k8D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide Free

IL-27/IL-35 EBI3 Subunit Antibody (15k8D10) - Azide Free Summary

Immunogen
A full-length human Interleukin-35-Fc fusion protein was used as immunogen for this antibody.
Specificity
The human Interleukin-35 protein is a dimeric protein consisting of the human IL-12a/p35 subunit (NP_000873.2) and the human EBI3 (NP_005746) protein. This clone has been tested to be specific to the EBI3 subunit of the target protein. Clone 15K8D10 tests as being specific to the EBI3 subunit.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EBI3
Purity
Protein G purified
Endotoxin Note
< 0.06 EU/ug
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 3-5ug/ml~
  • Immunohistochemistry-Paraffin 5ug/ml
Publications
Read Publications using
NBP2-27363 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Sterile - filtered PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for IL-27/IL-35 EBI3 Subunit Antibody (15k8D10) - Azide Free

  • cytokine receptor
  • EBI3
  • EBV-induced gene 3 protein
  • Epstein-Barr virus induced 3
  • Epstein-Barr virus induced gene 3
  • Epstein-Barr virus-induced gene 3 protein
  • IL-27 subunit beta
  • IL-27/IL-35 EBI3 Subunit
  • IL27B
  • IL-27B
  • IL35 EBI3
  • interleukin-27 subunit beta

Background

Interleukin-35 is a novel heterodimeric cytokine composed of the IL-12p35 subunit and the Epstein-Barr virus-induced gene 3 (EBI3) protein. As one of the IL-12 family members, Interleukin-35 is known to play an essential role in immune regulation of the CD4+CD25+ regulatory T (Treg) cells, from which Interleukin-35 is also specifically produced. Interleukin-35 is able to expand CD4+CD25+ Treg cells whereas it can inhibit proliferation of CD4+CD25- effector T (Teff) cells and development of proinflammatory Th17 cells, suppressing inflammatory responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-27/IL-35 EBI3 Subunit Antibody (NBP2-27363)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-27363AF405
Alexa Fluor 488 Labeled NBP2-27363AF488
Alexa Fluor 647 Labeled NBP2-27363AF647
Alexa Fluor 700 Labeled NBP2-27363AF700
Biotin Labeled NBP2-27363B
DyLight 350 Labeled NBP2-27363UV
DyLight 405 Labeled NBP2-27363V
DyLight 488 Labeled NBP2-27363G
DyLight 550 Labeled NBP2-27363R
DyLight 650 Labeled NBP2-27363C
DyLight 680 Labeled NBP2-27363FR
DyLight 755 Labeled NBP2-27363IR
HRP Labeled NBP2-27363H

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Bioinformatics

Gene Symbol EBI3
Entrez