IL-27/IL-35 EBI3 Subunit Antibody (10J706)

Images

 
Western Blot: EBI3 Antibody (10J706) [NBP2-03938] - Western blot testing of mouse EBI3 monoclonal antibody at 0.1 ug/ml on recombinant protein. Goat anti-rat Ig HRP secondary antibody and ECL substrate solution were ...read more
Flow Cytometry: IL-27/IL-35 EBI3 Subunit Antibody (10J706) [NBP2-03938] - EBI3 Antibody (10J706) [NBP2-03938] - Figure A: Intracellular analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow
Clone
10J706
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml
   

Biological Strategies

     

IL-27/IL-35 EBI3 Subunit Antibody (10J706) Summary

Immunogen
Full-length recombinant mouse EBI3 protein
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
EBI3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-3 ug/ml
  • Flow Cytometry 1-3ug/10^6 cells

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for IL-27/IL-35 EBI3 Subunit Antibody (10J706)

  • cytokine receptor
  • EBI3
  • EBV-induced gene 3 protein
  • Epstein-Barr virus induced 3
  • Epstein-Barr virus induced gene 3
  • Epstein-Barr virus-induced gene 3 protein
  • IL-27 subunit beta
  • IL-27/IL-35 EBI3 Subunit
  • IL27B
  • IL-27B
  • IL35 EBI3
  • interleukin-27 subunit beta

Background

EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively (Honglian Tong et al, 2010). IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-03938AF405
Alexa Fluor 488 Labeled NBP2-03938AF488
Alexa Fluor 647 Labeled NBP2-03938AF647
Alexa Fluor 700 Labeled NBP2-03938AF700
Allophycocyanin Labeled NBP2-03938APC
Biotin Labeled NBP2-03938B
DyLight 350 Labeled NBP2-03938UV
DyLight 405 Labeled NBP2-03938V
DyLight 488 Labeled NBP2-03938G
DyLight 550 Labeled NBP2-03938R
DyLight 650 Labeled NBP2-03938C
DyLight 680 Labeled NBP2-03938FR
DyLight 755 Labeled NBP2-03938IR
HRP Labeled NBP2-03938H
PE Labeled NBP2-03938PE
PerCP Labeled NBP2-03938PCP

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Bioinformatics

Gene Symbol EBI3
Entrez
Uniprot