IL-27 Antibody (DNT27)

Images

 
Western Blot: IL27 Antibody (DNT27) [NBP2-00454] - Mouse IL-27 Recombinant Protein (NBP1-43206) (lane 1) and mouse bone marrow-derived dendritic cells stimulated with LPS (lane 2) or left untreated (lane 3) were ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
DNT27
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

IL-27 Antibody (DNT27) Summary

Immunogen
The immunogen for this antibody is EBI3 (IL-27 subunit).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
IL27
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
Application Notes
This DNT27 antibody has been tested by western blot of cell lysates prepared from mouse EBI3-transfected 293T cells and bone marrow-derived dendritic cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for IL-27 Antibody (DNT27)

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A

Background

The monoclonal antibody DNT27 reacts with mouse Epstein-Barr Virus-induced gene 3 (EBI3). EBI3 is a 34 kDa soluble protein first identified in B cells that is upregulated after Epstein-Barr virus infection. EBI3 can exist as a homodimer and also heterodimerizes with IL-27A (p28, IL-30) to form the cytokine IL-27. IL-27 is produced most abundantly by dendritic cells and macrophages with some recent reports of expression in NKT cells. Recently, a complex of EBI3 and the p35 subunit of IL-12, called IL-35, has been reported to be produced by regulatory T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL27
Entrez