IL-27 Antibody (3H12.F10)


Western Blot: IL-27 Antibody (3H12.F10) [NBP2-19239] - Anti-IL-27/p28 antibody shows detection of recombinant mouse IL-27/p28. Recombinant protein was loaded on to an SDS-PAGE gel, and after separation, transferred to more
Flow Cytometry: IL-27 Antibody (3H12.F10) [NBP2-19239] - Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10ng/mL IFN? and 1ug/mL LPS for 14 hours and incubated for 4 hours with more

Product Details

Reactivity MuSpecies Glossary
Applications WB, ELISA, Flow
Please see the vial label for concentration. If unlisted please contact technical services.

IL-27 Antibody (3H12.F10) Summary

This antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Mature length recombinant mouse p28 protein (produced in E.coli). Protein accession number NP_663611.1.
IgG2a Kappa
Ion exchange chromatography
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
0.01% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Ion exchange chromatography


Application Notes
IL27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This antibody has been tested for use in western blotting. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.

Reactivity Notes

Reactive in Mouse.

Alternate Names for IL-27 Antibody (3H12.F10)

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-27 Antibody (NBP2-19239) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP2-19239AF405
Alexa Fluor (R) 488 Labeled NBP2-19239AF488
Alexa Fluor (R) 647 Labeled NBP2-19239AF647
Alexa Fluor (R) 700 Labeled NBP2-19239AF700
Allophycocyanin Labeled NBP2-19239APC
DyLight 350 Labeled NBP2-19239UV
DyLight 405 Labeled NBP2-19239V
DyLight 405LS Labeled NBP2-19239V3
DyLight 488 Labeled NBP2-19239G
DyLight 680 Labeled NBP2-19239FR
DyLight 755 Labeled NBP2-19239IR
PE Labeled NBP2-19239PE
PerCP Labeled NBP2-19239PCP

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Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25kD glycosylphosphatidylinositol (GPI)-linked differentiation antigen that is expressed by myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes express...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol IL27

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