IL-27 Antibody (3H12.F10)

Images

 
Western Blot: IL-27 Antibody (3H12.F10) [NBP2-19239] - Anti-IL-27/p28 antibody shows detection of recombinant mouse IL-27/p28. Recombinant protein was loaded on to an SDS-PAGE gel, and after separation, transferred to ...read more
Flow Cytometry: IL-27 Antibody (3H12.F10) [NBP2-19239] - Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10ng/mL IFN? and 1ug/mL LPS for 14 hours and incubated for 4 hours with ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, Flow
Clone
3H12.F10
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

IL-27 Antibody (3H12.F10) Summary

Description
This antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Mature length recombinant mouse p28 protein (produced in E.coli). Protein accession number NP_663611.1.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
IL27
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:10000
  • Flow Cytometry 1:10-1:1000
Application Notes
IL27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This antibody has been tested for use in western blotting. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.

Reactivity Notes

Reactive in Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for IL-27 Antibody (3H12.F10)

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IL-27 Antibody (NBP2-19239) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-19239AF405
Alexa Fluor 488 Labeled NBP2-19239AF488
Alexa Fluor 647 Labeled NBP2-19239AF647
Alexa Fluor 700 Labeled NBP2-19239AF700
Allophycocyanin Labeled NBP2-19239APC
DyLight 350 Labeled NBP2-19239UV
DyLight 405 Labeled NBP2-19239V
DyLight 488 Labeled NBP2-19239G
DyLight 680 Labeled NBP2-19239FR
DyLight 755 Labeled NBP2-19239IR
PE Labeled NBP2-19239PE
PerCP Labeled NBP2-19239PCP

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Blogs on IL-27.

Ly6G - a marker for monocytes, granulocytes and neutrophils
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25kD glycosylphosphatidylinositol (GPI)-linked differentiation antigen that is expressed by myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes express...  Read full blog post.

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Bioinformatics

Gene Symbol IL27
Entrez
Uniprot