IL-27 Antibody (3H12.F10)


Western Blot: IL-27 Antibody (3H12.F10) [NBP2-19239] - Anti-IL-27/p28 antibody shows detection of recombinant mouse IL-27/p28. Recombinant protein was loaded on to an SDS-PAGE gel, and after separation, transferred to more
Flow Cytometry: IL-27 Antibody (3H12.F10) [NBP2-19239] - Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10ng/mL IFN? and 1ug/mL LPS for 14 hours and incubated for 4 hours with more

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IL-27 Antibody (3H12.F10) Summary

This antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Anti-IL-27/p28 monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development.
IgG2a Kappa
Ion exchange chromatography
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  • Western Blot 1:1000
  • ELISA 1:10000
  • Flow Cytometry 1 ug / ml
Application Notes
IL27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This antibody has been tested for use in western blotting. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.

Reactivity Notes

Reactive in Mouse.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
1 mg/ml
Ion exchange chromatography

Alternate Names for IL-27 Antibody (3H12.F10)

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25kD glycosylphosphatidylinositol (GPI)-linked differentiation antigen that is expressed by myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes express...  Read full blog post.

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Gene Symbol IL27