IL-23A/IL-23 P19 Antibody (HLT2736)

Images

 
Western Blot: IL-23A/IL-23 P19 Antibody (HLT2736) [NBP1-52617] - Linker-free, recombinant human IL-23 was resolved by electrophoresis, transferred to nitrocellulose and probed with the anti-IL23 p19 specific antibody ...read more
Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody (HLT2736) [NBP1-52617] - Analysis of anti-IL-23 p19 antibody with human adrenal at concentration 10 ug/ml.
Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody (HLT2736) [NBP1-52617] - Analysis of anti-IL-23 p19 antibody with human lung, vasculature at concentration 10 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P
Clone
HLT2736
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IL-23A/IL-23 P19 Antibody (HLT2736) Summary

Immunogen
Recombinant human IL23A / IL-23.
Specificity
Human recombinant p40 and p19 with elastin linker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL23A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry-Paraffin 10 ug/ml
Theoretical MW
20.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IL-23A/IL-23 P19 Antibody (HLT2736)

  • IL23
  • IL-23
  • IL23A
  • IL-23A
  • IL-23-A
  • IL-23p19
  • IL23P19P19
  • interleukin 23 p19 subunit
  • interleukin 23, alpha subunit p19
  • interleukin-23 subunit alpha
  • Interleukin-23 subunit p19
  • JKA3 induced upon T-cell activation
  • MGC79388
  • SGRF
  • SGRFIL-23 subunit alpha

Background

The 473P19 antibody reacts with the p19 subunit of human IL-23. The 473P19 antibody was generated from immunization with authentic, insect cell-expressed, recombinant human IL-23 heterodimer. The use of a p19-specific capture antibody and a p40-specific detection antibody yields a human IL-23 sandwich ELISA exquisitely specific for human IL-23. IL-12 p40 monomer and IL-12 p70 were run in the assay at 200 ng/ml with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was also run in the IL-23 ELISA at 100 ng/ml with no cross reactivity observed. The assay has been validated by specific detection of significant levels of native human IL-23 protein in supernatants from a variety of different activated dendritic cell populations. IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-g. Mouse IL-23 does induce strong proliferation of memory T cells (but not nave T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12 human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-g production by nave and memory T cells, as compared to IL-12. IL-23-dependent, IL-17-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells. More recently, de novo differentiation of Th-17 cells in the absence of IL-23 has been demonstrated by treatment of nave CD4 cells with TGF-beta1 and IL-6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL23A
Entrez
Uniprot