Western Blot: IL-23 R Antibody [NB600-1147] - Analysis of IL-23R in (A) Jurkat and (B) NIH 3T3 cells. 1:500 dilution.
Immunohistochemistry-Paraffin: IL-23 R Antibody [NB600-1147] - Staining of IL-23 Receptor in Human periarterial lymphatic sheath lymphocytes (spleen) at 10 ug/ml. Hematoxylin-eosin counterstain.
Western Blot: IL-23 R Antibody [NB600-1147] - Analysis using IL-23R antibody. Lysate from (A) human Jurkat and (B) Mouse NIH 3T3 cells probed with IL-23R antibody at 0.5 ug/ml.
Immunohistochemistry-Paraffin: IL-23 R Antibody [NB600-1147] - Analysis of Mouse spleen tissue probed with isotype control antibody (top) and IL-23R antibody (bottom) at 5 ug/ml.
Simple Western: IL-23 R Antibody [NB600-1147] - Simple Western lane view shows a specific band for IL23 Receptor in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
Protein G purified
Based on immunogen sequence, this antibody will recognize the 629 amino acid (~68 KDa), 390 AA (~42 KDa) and 227 AA (~26 KDa) forms of human IL-23R. Flow (Cell Surface): See Kortylweski et al, 2009 for details. Use in Flow cell surface reported in scientific literature (PMID 24358364)In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB600-1147 in the following applications:
IL-23 is a heterodimeric cytokine that shares biological properties with proinflammatory cytokines. IL-23 is composed of the IL-12p40 soluble receptor subunit and a novel cytokine-like subunit related to IL-12p35, termed p19. IL-23 mediates it cellular function by binding to IL-23R, which pairs with IL-12Rb1 to confer IL-23 responsiveness. However, human IL-12 does not bind to human IL-23R. IL-23 has been shown to possess biological activities on T cells that are similar as well distinct from those of IL-12.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.