IL-23 R Antibody

Images

 
Western Blot: IL-23 R Antibody [NB600-1147] - Analysis of IL-23R in (A) Jurkat and (B) NIH 3T3 cells. 1:500 dilution.
Immunohistochemistry-Paraffin: IL-23 R Antibody [NB600-1147] - Staining of IL-23 Receptor in Human periarterial lymphatic sheath lymphocytes (spleen) at 10 ug/ml. Hematoxylin-eosin counterstain.
Western Blot: IL-23 R Antibody [NB600-1147] - Analysis using IL-23R antibody. Lysate from (A) human Jurkat and (B) Mouse NIH 3T3 cells probed with IL-23R antibody at 0.5 ug/ml.
Immunohistochemistry-Paraffin: IL-23 R Antibody [NB600-1147] - Analysis of Mouse spleen tissue probed with isotype control antibody (top) and IL-23R antibody (bottom) at 5 ug/ml.
Simple Western: IL-23 R Antibody [NB600-1147] - Simple Western lane view shows a specific band for IL23 Receptor in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, Flow-CS, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IL-23 R Antibody Summary

Immunogen
Synthetic peptide located at amino acids 439-455 (EIFIPEHKPTDYKKENT) of human IL-23R (NP_653302).
Localization
Cell Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL23R
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0 ug/ml
  • Simple Western 1:10
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Flow (Cell Surface)
Application Notes
Based on immunogen sequence, this antibody will recognize the 629 amino acid (approx. 68 KDa), 390 AA (approx. 42 KDa) and 227 AA (approx. 26 KDa) forms of human IL-23R. Flow (Cell Surface): See Kortylweski et al, 2009 for details. Use in Flow cell surface reported in scientific literature (PMID 24358364)

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Jurkat Lysate (NB800-PC2)
NIH 3T3 Lysate (NB800-PC8)
Publications
Read Publications using
NB600-1147 in the following applications:

  • 2 publications
  • IHC
    2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for IL-23 R Antibody

  • IBD17
  • IL-23 R
  • IL-23 receptor
  • IL23R
  • IL-23R
  • interleukin 23 receptor
  • interleukin-23 receptor

Background

IL-23 is a heterodimeric cytokine that shares biological properties with proinflammatory cytokines. IL-23 is composed of the IL-12p40 soluble receptor subunit and a novel cytokine-like subunit related to IL-12p35, termed p19. IL-23 mediates it cellular function by binding to IL-23R, which pairs with IL-12Rb1 to confer IL-23 responsiveness. However, human IL-12 does not bind to human IL-23R. IL-23 has been shown to possess biological activities on T cells that are similar as well distinct from those of IL-12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu
Applications: WB, Simple Western, Flow, Flow-CS, IHC, IHC-P
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Publications for IL-23 R Antibody (NB600-1147)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow-CS, IHC, WB.


Filter By Application
Flow-CS
(2)
IHC
(2)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
All Species
Showing Publications 1 - 5 of 5.
Publications using NB600-1147 Applications Species
Nakayama W, Jinnin M, Tomizawa Y et al. Dysregulated interleukin-23 signalling contributes to the increased collagen production in scleroderma fibroblasts via balancing microRNA expression. Rheumatology (Oxford) Oct 15 2016 [PMID: 27744356] (WB, IHC, Human) WB, IHC Human
Walker CR, Hautefort I, Dalton JE, et al. Intestinal Intraepithelial Lymphocyte-Enterocyte Crosstalk Regulates Production of Bactericidal Angiogenin 4 by Paneth Cells upon Microbial Challenge. PLoS One 2013 Dec 17 [PMID: 24358364] (Flow-CS, Mouse) Flow-CS Mouse
Wang S, Xu X, Xie A et al. Anti-interleukin-12/23p40 antibody attenuates chronic rejection of cardiac allografts partly via inhibition gamma delta T cells Clin Exp Immunol 2012 Sep [PMID: 22861372] (IHC, Mouse) IHC Mouse
Li Z, Wu F, Brant SR, Kwon JH. IL-23 receptor regulation by Let-7f in human CD4+ memory T cells. J Immunol. 2011 Jun 1 [PMID: 21508257] (WB, Human)

Details:
IL-23 Receptor (IMG-5092A). WB: Human primary memory T cells transfected with control or Let-7f, Fig 6.
WB Human
Kortylewski M, Xin H, Kujawski M et al. Regulation of the IL-23 and IL-12 balance by Stat3 signaling in the tumor microenvironment. Cancer Cell. 2009 Feb 3 [PMID: 19185846] (Flow-CS, Mouse)

Details:
IL-23R (IMG-5092A): Flow (cell surface), mouse spleen and lymph nodes including lymph nodes from tumor models, Fig 5A. Prior to staining, freshly isolated cells were preincubated with an FcgammaIII/IIR-specific antibody to block non-specific binding. The
Flow-CS Mouse

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IL23R
Entrez
Uniprot