IL-22 R alpha 1 Antibody (496514)

Images

 
IL‑22 R alpha 1 was detected in immersion fixed Hepa 1‑6 mouse hepatoma cell line using Rat Anti-Mouse IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB42941) at 10 µg/mL for 3 hours at room temperature. Cells ...read more
Hepa 1‑6 mouse hepatoma cell line was stained with Rat Anti-Mouse IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB42941, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready, ICC
Clone
496514
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

IL-22 R alpha 1 Antibody (496514) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-22 R alpha 1
Thr18-Ala228
Accession # Q80XZ4
Specificity
Detects mouse IL-22 R alpha 1 in direct ELISAs. In direct ELISAs, 25% cross-reactivity with recombinant human (rh) IL‑22 R alpha 1 is observed and no cross-reactivity with rhIL-20 R alpha, recombinant mouse (rm) IL-20 R alpha, rhIL-22BP, or rmIL-22BP is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
IL22RA1
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB42941 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-22 R alpha 1 Antibody (496514)

  • CRF2-9
  • CRF2-9interleukin 22 receptor
  • Cytokine receptor class-II member 9
  • Cytokine receptor family 2 member 9
  • IL-22 R alpha 1
  • IL-22 receptor subunit alpha-1
  • IL22R alpha 1
  • IL22R
  • IL22R1
  • IL22RA1
  • IL-22Ra1
  • IL-22R-alpha-1
  • IL-TIF-R1
  • interleukin 22 receptor, alpha 1
  • interleukin-22 receptor subunit alpha-1
  • zcytoR11

Background

The IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa type I transmembrane glycoprotein that belongs to the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 330 aa cytoplasmic domain (1). Within the ECD, mouse IL-22 R alpha 1 shares 78%, 78%, and 94% aa sequence identity with canine, human, and rat IL-22 R alpha 1, respectively. It shares 20%‑26% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22, and -24 receptors (2). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (3, 4), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (5, 6). In both cases, IL‑22 R alpha 1 functions as the high affinity ligand binding subunit, and subsequent association with IL-10 R beta or IL-20 R beta serves to stabilize the complex (3, 6‑9). IL‑22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but association with IL-10 R beta or IL-20 R beta is required for signal transduction (3, 7). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (8, 10). Even though it is a receptor for interleukins, IL‑22 R alpha 1 is not expressed on hematopoietic cells (7, 11, 12). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (7, 11‑14). IL‑22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (4, 12, 14, 15). IL‑22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (4, 15).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-22 R alpha 1 Antibody (MAB42941)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow, ICC Fresh, IHC, IHC Frozen.


Filter By Application
Flow
(3)
ICC Fresh
(1)
IHC
(2)
IHC Frozen
(1)
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Mouse
(7)
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Showing Publications 1 - 7 of 7.
Publications using MAB42941 Applications Species
YH Hsu, HH Li, JM Sung, WY Chen, YC Hou, YH Weng, WT Lai, CH Wu, MS Chang Interleukin-20 targets podocytes and is upregulated in experimental murine diabetic nephropathy Exp. Mol. Med., 2017;49(3):e310. 2017 [PMID: 28360429] (ICC Fresh,, Mouse) ICC Fresh Mouse
P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection Mucosal Immunol, 2017;0(0):. 2017 [PMID: 28247861] (IHC,, Mouse) IHC Mouse
A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice Nat Commun, 2016;7(0):13896. 2016 [PMID: 27982034] (Flow,, Mouse) Flow Mouse
Justa S, Zhou X, Sarkar S Endogenous IL-22 plays a dual role in arthritis: regulation of established arthritis via IFN-gamma responses. PLoS ONE, 2014;9(3):e93279. 2014 [PMID: 24676270] (Flow,, Mouse) Flow Mouse
Kumar P, Thakar M, Ouyang W, Malarkannan S IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection. Mucosal Immunol, 2013;6(1):69-82. 2013 [PMID: 22739232] (IHC Frozen,, Mouse) IHC Frozen Mouse
Li Z, Burns AR, Miller SB, Smith CW CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing. FASEB J., 2011;25(8):2659-68. 2011 [PMID: 21518851] (Flow,, Mouse) Flow Mouse
Lindroos J, Svensson L, Norsgaard H IL-23-Mediated Epidermal Hyperplasia Is Dependent on IL-6. J. Invest. Dermatol., 2011;131(5):1110-8. 2011 [PMID: 21289639] (IHC,, Mouse) IHC Mouse

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Bioinformatics

Gene Symbol IL22RA1
Entrez
Uniprot