IL-22 R alpha 1 Antibody (305405)

Images

 
COLO 205 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB2770, filled histogram) or isotype control antibody (Catalog # MAB002, open ...read more
IL‑22 R alpha 1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line using Mouse Anti-Human IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB2770) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ICC
Clone
305405
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

IL-22 R alpha 1 Antibody (305405) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha 1
Pro18-Thr228
Accession # Q8N6P7
Specificity
Detects human IL‑22 R alpha 1 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) IL-10, rhIL-22BP or rhIL-20 R alpha is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL22RA1
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB2770 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • 1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-22 R alpha 1 Antibody (305405)

  • CRF2-9
  • CRF2-9interleukin 22 receptor
  • Cytokine receptor class-II member 9
  • Cytokine receptor family 2 member 9
  • IL-22 R alpha 1
  • IL-22 receptor subunit alpha-1
  • IL22R alpha 1
  • IL22R
  • IL22R1
  • IL22RA1
  • IL-22Ra1
  • IL-22R-alpha-1
  • IL-TIF-R1
  • interleukin 22 receptor, alpha 1
  • interleukin-22 receptor subunit alpha-1
  • zcytoR11

Background

IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL‑22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta  is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding IL‑22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22%‑25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-22 R alpha 1 Antibody (MAB2770)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow, IHC, Neut, WB.


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Flow
(1)
IHC
(1)
Neut
(1)
WB
(3)
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Human
(5)
Mouse
(1)
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Showing Publications 1 - 6 of 6.
Publications using MAB2770 Applications Species
P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection Mucosal Immunol, 2017;0(0):. 2017 [PMID: 28247861] (IHC,, Mouse) IHC Mouse
Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes. J Neuroinflammation, 2015;12(0):119. 2015 [PMID: 26077779] (Flow,, Human) Flow Human
Lerman, Galya, Sharon, Moran, Leibowitz-Amit, Raya, Sidi, Yechezke, Avni, Dror The crosstalk between IL-22 signaling and miR-197 in human keratinocytes. PLoS ONE, 2014;9(9):e107467. 2014 [PMID: 25208211] (WB,, Human) WB Human
Dash R, Bhoopathi P, Das S, Sarkar S, Emdad L, Dasgupta S, Sarkar D, Fisher P Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction. Cancer Res, 0;74(2):563-74. 0 [PMID: 24282278] (WB,, Human) WB Human
Zhang F, Shang D, Zhang Y, Tian Y Interleukin-22 suppresses the growth of A498 renal cell carcinoma cells via regulation of STAT1 pathway. PLoS ONE, 2011;6(5):e20382. 2011 [PMID: 21625390] (WB,, Human) WB Human
Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH TH17 cytokines induce human airway smooth muscle cell migration. J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2. 2011 [PMID: 21345484] (Neut,, Human) Neut Human

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Bioinformatics

Gene Symbol IL22RA1
Entrez
Uniprot