IL-2 Recombinant Protein


SDS-Page: IL2 Protein [NBC1-18462] - Interleukin-2(C145S), 15.4kDa (133aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Reactivity HuSpecies Glossary
Applications PAGE
1.0 mg/ml

Order Details

IL-2 Recombinant Protein Summary

A recombinant protein corresponding to IL2.


Details of Functionality
Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1.5 ng/ml.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Liquid. In Phosphate buffer saline (pH 7.4)
No Preservative
1.0 mg/ml


Application Notes
Cell line: CTLL2 (mouse cytotoxic Tcell)
Maintenance Condition: 10% FBS RPMI 1640 with hIL2
Assay medium: 10% FBS RPMI 1640 without hIL2
Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul)
Starvation: 24hr, Assay medium
Incubation Time: 24hr (after sample treatment)
Concentration Range: 0.039 ng/ml - 40 ng/ml
Detection method: MTT assay

Reactivity Notes



The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 15.4kDa (133aa), confirmed by MALDI-TOF.

Alternate Names for IL-2 Recombinant Protein

  • aldesleukin
  • IL2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF


IL-2, also known as T cell growth factor(TCGF), is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. This recombinant human IL-2 is a 15.4 kDa protein, containing 133 amino acid residues including one intra chain disulfide bond. Recombinant human IL-2 was overexpressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product PDFs


Gene Symbol IL2

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