IL-2 Antibody [HRP]

Images

 
Western Blot: IL-2 Antibody [HRP] [NBP1-77570] - Lane 1: 2ug Human IL-2 Recombinant Protein. Lane 2: 1ug Hu IL-2 Recombinant Protein. Lane 3: 500ng Hu IL-2 Recombinant Protein. Lane 4: 250ng Hu IL-2 Recombinant Protein. ...read more
Western Blot: IL-2 Antibody [HRP] [NBP1-77570] - Western Blot of Rabbit anti-IL-2 Antibody Peroxidase Conjugated Lane 1: Human IL-2 Recombinant Protein Load: 50 ng per lane Secondary antibody: Peroxidase Conjugated IL-2 ...read more
Western Blot: IL-2 Antibody [HRP] [NBP1-77570] - Lane 1: 250ng glycosylated IL-2. Lane 2: 250ng non-glycosylated IL-2. Secondary antibody: Peroxidase Conjugated IL-2 Antibody at 1:10,000 for 60 min at RT. Lane 3: 250ng ...read more
Western Blot: IL-2 Antibody [HRP] [NBP1-77570] - Lane 1: 250ng glycosylated IL-2. Lane 2: 250ng non-glycosylated IL-2. Secondary antibody: Peroxidase Conjugated IL-2 Antibody at 1:1000 for 60 min at RT. Block: TBST + 5% ...read more

Product Details

Summary
Product Discontinued
View other related IL-2 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-77570
    • Availability
      Product Discontinued

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IL-2 Antibody [HRP] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-2 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
IL2
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 1:500-1:2500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
By western blot a band approximately 15 kDa in size corresponding - human IL-2 protein is expected in the appropriate cell lysate or extract.
Readout System

Reactivity Notes

Reactive in Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Delipidation and Defibrination
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water (or equivalent) to desired concetration.

Alternate Names for IL-2 Antibody [HRP]

  • aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

CD122 is a 70-75 kD IL-2 receptor beta chain also known as IL-2Rbeta, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rbeta chain can combine with either the common gamma subunit (gamma-c, CD132) alone or with the gamma-c subunit and the IL-2Ralpha subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for IL-2 Antibody (NBP1-77570)

Discover related pathways, diseases and genes to IL-2 Antibody (NBP1-77570). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for IL-2 Antibody (NBP1-77570)

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Research Areas for IL-2 Antibody (NBP1-77570)

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Bioinformatics

Gene Symbol IL2
Entrez