IL-2 Antibody (5H4)

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Flow Cytometry: IL-2 Antibody (5H4) [NBP1-49047] - C57BL/6 mouse splenocytes stained with 5H4 PE and CD3 APC

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-49047
    • Availability
      Product Discontinued

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IL-2 Antibody (5H4) Summary

Immunogen
This antibody was generated by immunizing host with Rat myeloma YB2/0 transfected with truncated mouse Il-2Rβ cDNA
Localization
T and B cells, NK cells, monocytes, macrophages
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
IL2
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for IL-2 Antibody (5H4)

  • aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

CD122 is a 70-75 kD IL-2 receptor beta chain also known as IL-2Rbeta, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rbeta chain can combine with either the common gamma subunit (gamma-c, CD132) alone or with the gamma-c subunit and the IL-2Ralpha subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for IL-2 Antibody (NBP1-49047)

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Bioinformatics

Gene Symbol IL2
Entrez