IL-18 R alpha/IL-1 R5 Antibody

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In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready, Neut
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

IL-18 R alpha/IL-1 R5 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha /IL-1 R5
Ser19-Gly326
Accession # Q61098
Specificity
Detects mouse IL‑18 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human IL‑18 R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Il18rap
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Neutralization 0.2-0.8 ug/mL
Publications
Read Publications using
AF856 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18 R alpha/IL-1 R5 Antibody

  • CD218a
  • IL-1 R5
  • IL-1 Rrp
  • IL-18 R alpha
  • IL-18 R1
  • IL18R alpha
  • IL18R1
  • IL-18Ra

Background

Interleukin-18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL18 in vitro, coexpression of IL-18 R alpha and IL18 R beta is required for high affinity binding and IL-18 responsiveness.

Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-18 R alpha/IL-1 R5 Antibody (AF856)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Flow, IHC Frozen, Neut.


Filter By Application
Flow
(3)
IHC Frozen
(2)
Neut
(2)
All Applications
Filter By Species
Mouse
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF856 Applications Species
Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). J. Biol. Chem., 2009;0(0):. 2009 [PMID: 19592492] (Neut,, Mouse) Neut Mouse
Wiener Z, Pocza P, Racz M, Nagy G, Tolgyesi G, Molnar V, Jaeger J, Buzas E, Gorbe E, Papp Z, Rigo J, Falus A IL-18 induces a marked gene expression profile change and increased Ccl1 (I-309) production in mouse mucosal mast cell homologs. Int. Immunol., 2008;20(12):1565-73. 2008 [PMID: 18940934] (Flow,, Mouse) Flow Mouse
Andoh T, Kishi H, Motoki K, Nakanishi K, Kuraishi Y, Muraguchi A Protective effect of IL-18 on kainate- and IL-1beta-induced cerebellar ataxia in mice. J. Immunol., 2008;180(4):2322-8. 2008 [PMID: 18250441] (IHC Frozen,, Mouse) IHC Frozen Mouse
Chandrasekar B, Patel DN, Mummidi S, Kim JW, Clark RA, Valente AJ Interleukin-18 suppresses adiponectin expression in 3T3-L1 adipocytes via a novel signal transduction pathway involving ERK1/2-dependent NFATc4 phosphorylation. J. Biol. Chem., 2007;283(7):4200-9. 2007 [PMID: 18086672] (Flow,, Mouse) Flow Mouse
Chaudhry UI, Kingham TP, Plitas G, Katz SC, Raab JR, DeMatteo RP Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth. Cancer Res., 2006;66(21):10497-504. 2006 [PMID: 17079471] (Flow,, Mouse) Flow Mouse
Wang X, Hagberg H, Mallard C, Zhu C, Hedtjarn M, Tiger CF, Eriksson K, Rosen A, Jacobsson B Disruption of interleukin-18, but not interleukin-1, increases vulnerability to preterm delivery and fetal mortality after intrauterine inflammation. Am. J. Pathol., 2006;169(3):967-76. 2006 [PMID: 16936270] (IHC Frozen,, Mouse) IHC Frozen Mouse
Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis. J. Immunol., 2004;172(3):1754-62. 2004 [PMID: 14734758] (Neut,, Mouse) Neut Mouse

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Bioinformatics

Gene Symbol Il18rap
Entrez
Uniprot