IL-18 R alpha/IL-1 R5 Antibody

Images

 
Recombinant Human IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line, in a dose-dependent manner (orange line), as measured by the Human ...read more
IL‑18 R alpha /IL‑1 R5 was detected in immersion fixed frozen sections of human skin using Goat Anti-Human IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, Neut
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

IL-18 R alpha/IL-1 R5 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
Glu20-Arg329
Accession # Q13478
Specificity
Detects human IL‑18 R alpha /IL-1 R5 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL‑18 R alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL18R1
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Neutralization 0.1-0.4 ug/mL
Publications
Read Publications using
AF840 in the following applications:

  • ICC
    1 publication
  • IF
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18 R alpha/IL-1 R5 Antibody

  • CD218a
  • IL-1 R5
  • IL-1 Rrp
  • IL-18 R alpha
  • IL-18 R1
  • IL18R alpha
  • IL18R1
  • IL-18Ra

Background

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL‑18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate, and placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-18 R alpha/IL-1 R5 Antibody (AF840)(5)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ICC, IF, WB.


Filter By Application
ICC
(1)
IF
(1)
WB
(3)
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Human
(4)
Rat
(1)
All Species

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Bioinformatics

Gene Symbol IL18R1
Entrez
Uniprot