IL-18 R alpha/IL-1 R5 Antibody (70625) [Unconjugated]

Images

 
IL‑18 R alpha /IL‑1 R5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human IL‑18 R alpha /IL‑1 R5 Mono­clonal Antibody (Catalog # MAB840) for 3 ...read more
In the presence of Recombinant Human TNF‑ alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL‑18/IL‑1F4 (Catalog # B003-5) stimulates IFN‑ gamma secretion in KG-1 human myeloid leukemia cells in a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready, ICC/IF
Clone
70625
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

IL-18 R alpha/IL-1 R5 Antibody (70625) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
Glu20-Arg329
Accession # Q13478
Specificity
Detects Recombinant Human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL18R1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 0.03-0.1 ug/mL
Publications
Read Publications using
MAB840 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18 R alpha/IL-1 R5 Antibody (70625) [Unconjugated]

  • CD218a
  • IL-1 R5
  • IL-1 Rrp
  • IL-18 R alpha
  • IL-18 R1
  • IL18R alpha
  • IL18R1
  • IL-18Ra

Background

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

  1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
  2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-18 R alpha/IL-1 R5 Antibody (MAB840)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Flow, IHC Frozen, IHC Paraffin-embedded, Neut, WB.


Filter By Application
Flow
(4)
IHC Frozen
(1)
IHC Paraffin-embedded
(1)
Neut
(2)
WB
(1)
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Human
(9)
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Showing Publications 1 - 9 of 9.
Publications using MAB840 Applications Species
Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma Sci Rep, 2016;6(0):26757. 2016 [PMID: 27225603] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Wang J, Sun C, Gerdes N, Liu C, Liao M, Liu J, Shi M, He A, Zhou Y, Sukhova G, Chen H, Cheng X, Kuzuya M, Murohara T, Zhang J, Cheng X, Jiang M, Shull G, Rogers S, Yang C, Ke Q, Jelen S, Bindels R, Ellison D, Jarolim P, Libby P, Shi G Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter. Nat Med, 2015;21(7):820-6. 2015 [PMID: 26099046] (IHC Frozen,, Human) IHC Frozen Human
Li W, Okuda A, Yamamoto H, Yamanishi K, Terada N, Yamanishi H, Tanaka Y, Okamura H Regulation of development of CD56 bright CD11c + NK-like cells with helper function by IL-18. PLoS ONE, 2013;8(12):e82586. 2013 [PMID: 24376549] (Flow,, Human) Flow Human
Blom L, Poulsen L IL-1 Family Members IL-18 and IL-33 Upregulate the Inflammatory Potential of Differentiated Human Th1 and Th2 Cultures. J Immunol, 2012;189(9):4331-7. 2012 [PMID: 23028054] (Neut,, Human) Neut Human
Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis. Arthritis Res. Ther., 2011;13(2):R52. 2011 [PMID: 21435242] (WB,, Human) WB Human
Tucci M, Quatraro C, Lombardi L, Pellegrino C, Dammacco F, Silvestris F Glomerular accumulation of plasmacytoid dendritic cells in active lupus nephritis: role of interleukin-18. Arthritis Rheum., 2008;58(1):251-62. 2008 [PMID: 18163476] (Flow,, Human) Flow Human
Rao DA, Tracey KJ, Pober JS IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response. J. Immunol., 2007;179(10):6536-46. 2007 [PMID: 17982042] (Neut,, Human) Neut Human
Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X CD molecules 2006--human cell differentiation molecules. J. Immunol. Methods, 2007;319(1):1-5. 2007 [PMID: 17174972] (Flow,, Human) Flow Human
Ebert EC, Mehta V Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Cell. Immunol., 2006;241(1):7-13. 2006 [PMID: 16914126] (Flow,, Human) Flow Human

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Bioinformatics

Gene Symbol IL18R1
Entrez
Uniprot