IL-18/IL-1F4 Recombinant Protein


SDS-Page: IL18 Protein [NBC1-18463] - Interleukin-18, 18.3kDa (158aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Reactivity HuSpecies Glossary
Applications PAGE
1.0 mg/ml

Order Details

IL-18/IL-1F4 Recombinant Protein Summary

A recombinant protein corresponding to IL18.


Details of Functionality
Measured by its ability to induce IFN-gamma secretion using KG-1 human myeloid leukemia cells in the presence of TNF-alpha (20 ng/ml). The ED50 for this effect is < 0.85 ug/ml.
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Liquid. In Phosphate buffer saline (pH 7.4).
No Preservative
1.0 mg/ml


Application Notes
Cell line: KG-1 (human myeloid leukemia cell)
Maintenance condition: 20% FBS RPMI 1640
Assay medium: 1% FBS RPMI 1640 with TNF-alpha 20 ng/ml
Cell density: 3 x 104 cells/well (96 well plate)
Incubation time: 24hr (after sample treatment)
Concentration range: 300 pg/ml - 10 ug/ml
Detection method: IFN-gamma ELISA Kit (BD biosciences, Cat. No. 555142). Use in In-vivo reported in scientific literature (PMID 26193793)
Read Publication using NBC1-18463.


The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 18.3kDa (158aa), confirmed by MALDI-TOF.

Alternate Names for IL-18/IL-1F4 Recombinant Protein

  • Iboctadekin
  • IFN-gamma-inducing factor
  • IGIF
  • IGIFIL-1 gamma
  • IL18
  • IL-18MGC12320
  • IL-1F4
  • IL1F4iboctadekin
  • IL-1g
  • Interferon gamma-inducing factor
  • interleukin 18 (interferon-gamma-inducing factor)
  • Interleukin-1 gamma
  • interleukin-18


Interleukin 18 (IL-18), previously known as interferon-gamma (IFN-gamma)-inducing factor, is an activator of NK cells and a co-inducer of Th1 cytokines. IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IL-18 was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for IL-18/IL-1F4 Recombinant Protein (NBC1-18463)(1)

We have publications tested in 1 application: In vivo.

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Contact Information

Product PDFs


Gene Symbol IL18

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