Recombinant Human IL-18/IL-1F4 Protein

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SDS-Page: IL18 Protein [NBC1-18463] - Interleukin-18, 18.3kDa (158aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human IL-18/IL-1F4 Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 37-193 of Human IL-18/IL-1F4

Source: E.coli

Amino Acid Sequence: MYFGKLESKL SVIRNLNDQV LFIDQGNRPL FEDMTDSDCR DNAPRTIFII SMYKDSQPRG MAVTISVKCE KISTLSCENK IISFKEMNPP DNIKDTKSDI IFFQRSVPGH DNKMQFESSS YEGYFLACEK ERDLFKLILK KEDELGDRSI MFTVQNED

Details of Functionality
Measured by its ability to induce IFN-gamma secretion using KG-1 human myeloid leukemia cells in the presence of TNF-alpha. The ED50 for this effect is less or equal to 850 ng/ml.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
IL18
Purity
>95% pure by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Application Notes
Use in In-vivo reported in scientific literature (PMID 26193793)
Theoretical MW
18.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBC1-18463.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffer saline (pH 7.4)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human IL-18/IL-1F4 Protein

  • Iboctadekin
  • IFN-gamma-inducing factor
  • IGIF
  • IGIFIL-1 gamma
  • IL18
  • IL-18
  • IL-18MGC12320
  • IL-1F4
  • IL1F4iboctadekin
  • IL-1g
  • Interferon gamma-inducing factor
  • interleukin 18 (interferon-gamma-inducing factor)
  • Interleukin-1 gamma
  • interleukin-18

Background

Interleukin 18 (IL-18), previously known as interferon-gamma (IFN-gamma)-inducing factor, is an activator of NK cells and a co-inducer of Th1 cytokines. IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IL-18 was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Publications for IL-18/IL-1F4 Recombinant Protein (NBC1-18463)(1)

We have publications tested in 1 application: In vivo.


Filter By Application
In vivo
(1)
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Bioinformatics

Gene Symbol IL18
Entrez