IL-17F Antibody (H17F10A7)

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Western Blot: IL-17F Antibody (H17F10A7) [NBP1-43234] - IL-17 producing cells were generated by culturing human PBMC with anti-Human CD3 and anti-Human CD28 in the presence of Human IL-23 Recombinant for 3 days, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
H17F10A7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IL-17F Antibody (H17F10A7) Summary

Immunogen
The immunogen for this antibody was IL17F.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL17F
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This H17F10A7 antibody has been tested at 1-2ug/ml by western blot analysis of cultured, activated Th17 cells, where it detects a band of approximately 18kDa.It is recommended that H17F10A7 be used under reducing conditions only.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for IL-17F Antibody (H17F10A7)

  • Cytokine ML-1
  • IL17F
  • IL-17F
  • IL-17Finterleukin-17F
  • IL24
  • IL-24
  • interleukin 17F
  • Interleukin-24
  • ML-1
  • ML1interleukin-24

Background

The monoclonal antibody H17F10A7 reacts with human IL-17F, a member of the IL-17 family of pro-inflammatory cytokines. Like IL-17A, IL-17F is a disulfide-linked, homodimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found also in the TGF-, BMP, and NGF superfamilies. The presence of the cysteine knot motif suggested the possibility of a heterodimeric structure, as was reported for TGF- and inhibin/activin. Recent reports confirm that co-expression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Moreover, activated human CD4+ T cells were found to produce the IL-17A/F heterodimer, along with the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). These heterodimers can be detected by immunoprecipitation with anti-IL-17A monoclonal antibody followed by immunoblot with H17F10A7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL17F
Entrez