Human IL-17AF ELISA Kit (Colorimetric) Summary
Measures free human IL-17AF in the supernatant of activated cells.
|Standard Curve Range
ELISA Kit (Colorimetric)
For the detection of human IL-17AF in the supernatant. The human IL-17AF ActivELISA(TM) Kit measures free IL-17AF in the supernatant of activated cells. Standard protocols for detecting IL-17AF activity include ELISA and Western Blot. The IL-17AF ActivELISA(TM) can be completed in one day using a sandwich ELISA protocol. The anti-IL-17F antibody is coated on a plate which then captures free IL-17AF. The amount of bound IL-17AF is detected by adding a biotinylated anti-IL-17A antibody followed by HRP-conjugated streptavidin. ABTS substrate is then added and the concentration is determined by colorimetric detection in an ELISA plate reader.
Packaging, Storage & Formulations
Storage is content dependent.
- Coating Buffer 25 ml
- BSA 4 x 0.5 g
- ELISA Strip 96 Well Plates 2
- 100X Capture Antibody* 250 ul
- 10X Blocking Buffer 20 ml
- 100X Detection Antibody* 250 ul
- 20X Wash Buffer 50 ml
- ABTS Substrate 2 x 12 ml
- Recombinant IL-17AF Standard 1 vial
- HRP-Conjugated Streptavidin 10 ul
Additional items required: Distilled water 96-well ELISA plate reader
Alternate Names for Human IL-17AF ELISA Kit (Colorimetric)
- interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
- interleukin 17A
- interleukin 17F
Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by a subset of T helper cells that develops distinct from the Th1- and Th2- cell differentiation pathways. IL-17, also known as CTLA-8, stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGFB (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other proinflammatory cytokines. IL-17 (~32kDa) protein is produced and exists as a homo-dimer, has homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterized and highly expressed by activated effector memory T cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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