Human IL-17AF ELISA Kit (Colorimetric)

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ELISA: Human IL-17AF ELISA Kit [NBP2-31047] - Standard curve using the human IL-17AF standard.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell supernatant
Standard Curve Range
62.5-4000 pg/ml
Sensitivity
62.5 pg/ml

Order Details

Human IL-17AF ELISA Kit (Colorimetric) Summary

Specificity
Measures free human IL-17AF in the supernatant of activated cells.
Standard Curve Range
62.5-4000 pg/ml
Sensitivity
62.5 pg/ml
Assay Type
Sandwich ELISA
Sample Volume
0.1 ml
Kit Type
ELISA Kit (Colorimetric)
Gene
IL17

Applications/Dilutions

Application Notes
For the detection of human IL-17AF in the supernatant. The human IL-17AF ActivELISA(TM) Kit measures free IL-17AF in the supernatant of activated cells. Standard protocols for detecting IL-17AF activity include ELISA and Western Blot. The IL-17AF ActivELISA(TM) can be completed in one day using a sandwich ELISA protocol. The anti-IL-17F antibody is coated on a plate which then captures free IL-17AF. The amount of bound IL-17AF is detected by adding a biotinylated anti-IL-17A antibody followed by HRP-conjugated streptavidin. ABTS substrate is then added and the concentration is determined by colorimetric detection in an ELISA plate reader.

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Recombinant IL-17AF Standard 1 vial
  2. 100X Capture Antibody* 250 ul
  3. 100X Detection Antibody* 250 ul
  4. 10X Blocking Buffer 20 ml
  5. 20X Wash Buffer 50 ml
  6. ABTS Substrate 2 x 12 ml
  7. Coating Buffer 25 ml
  8. BSA 4 x 0.5 g
  9. ELISA Strip 96 Well Plates 2
  10. HRP-Conjugated Streptavidin 10 ul

Notes

Additional items required: Distilled water 96-well ELISA plate reader

Alternate Names for Human IL-17AF ELISA Kit (Colorimetric)

  • IL17
  • IL-17
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A
  • interleukin 17F

Background

Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by a subset of T helper cells that develops distinct from the Th1- and Th2- cell differentiation pathways. IL-17, also known as CTLA-8, stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGFB (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other proinflammatory cytokines. IL-17 (~32kDa) protein is produced and exists as a homo-dimer, has homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterized and highly expressed by activated effector memory T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL17