IL-17AF ELISA Kit Summary
Packaging, Storage & Formulations
Storage is content dependent.
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
For the detection of human IL-17AF in the supernatant
The human IL-17AF ActivELISATM Kit measures free IL-17AF in the supernatant
of activated cells. Standard protocols for detecting IL-17AF activity
include ELISA and Western Blot. The IL-17AF ActivELISATM can be completed
in one day using a sandwich ELISA protocol. The anti-IL-17F antibody is
coated on a plate which then captures free IL-17AF. The amount of bound
IL-17AF is detected by adding a biotinylated anti-IL-17A antibody followed
by HRP-conjugated streptavidin. ABTS substrate is then added and the concentration
is determined by colorimetric detection in an ELISA plate reader.
- 100X Capture Antibody* 250 ul
- 100X Detection Antibody* 250 ul
- BSA 4 x 0.5 g
- 20X Wash Buffer 50 ml
- Coating Buffer 25 ml
- 10X Blocking Buffer 20 ml
- HRP-Conjugated Streptavidin 10 ul
- ABTS Substrate 2 x 12 ml
- Recombinant IL-17AF Standard 1 vial
- ELISA Strip 96 Well Plates 2
- Manual 1
Additional items required:
96-well ELISA plate reader
Alternate Names for IL-17AF ELISA Kit
- interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
- interleukin 17A
- interleukin 17F
Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by a subset
of T helper cells that develops distinct from the Th1- and Th2- cell differentiation
pathways. IL-17, also known as CTLA-8, stimulates induction of other
pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports
strongly suggest the involvement of IL-17 in several chronic inflammatory
diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGFB
(differentiation) and IL-23 (expansion) are required for induction and maintenance
of Th17 (IL-17 producing) cells, which in turn induce the other proinflammatory
cytokines. IL-17 (~32kDa) protein is produced and exists as a
homo-dimer, has homology to a herpes virus early protein, is one of the six
members (IL-17A-F) of this cytokine family, and is well characterized and
highly expressed by activated effector memory T cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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Product General Protocols
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