IL-17 RD/SEF Antibody

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IL‑17 RD/SEF was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Goat Anti-Human IL‑17 RD/SEF Antigen Affinity-purified Polyclonal Antibody ...read more
K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human IL‑17 RD/SEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2275, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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IL-17 RD/SEF Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑17 RD/SEF
Ala27-Arg299
Accession # AAM77571
Specificity
Detects human IL‑17 RD/SEF in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) IL-17 RD is observed and less than 10% cross-reactivity with recombinant human (rh) IL-17 RC, rmIL-17B R, and rhIL-17 R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL17RD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 1 Review rated 4
using
AF2275 in the following applications:

Publications
Read Publication using
AF2275 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17 RD/SEF Antibody

  • FLJ35755
  • IL-17 RD
  • IL17RD
  • IL-17RD
  • IL-17RDIL-17 receptor D
  • IL17Rhom
  • IL17RLM
  • IL17RLMDKFZp434N1928
  • interleukin 17 receptor D
  • interleukin-17 receptor D
  • Interleukin-17 receptor-like protein
  • MGC133309
  • Sef homolog
  • SEF
  • SEFhSef

Background

Interleukin-17 receptor D (IL-17 RD), also known as SEF (similar expression to FGFs), is a type I transmembrane protein that is found in both the cytoplasm and plasma membrane (1-5). The gene for this protein belongs to a synexpression group originally identified in zebrafish where SEF is expressed along with FGF-3, -8, sprouty-2 (SPRY2) and SPRY4 (6, 7). By alternate splicing, two transcript variants, potentially encoding three protein isoforms, exist. One is a full-length long form, one a shortened form that uses an alternate start site, and one an alternate splice form that removes the classic signal sequence (1-4). These isoforms have different expression patterns, subcellular localization, and function. The membrane-bound long form of human IL-17RD is synthesized as a 739 amino acid (aa) precursor protein with a putative 27 aa signal peptide, a 272 aa extracellular domain, a 20 aa transmembrane segment and a 420 aa cytoplastic domain. The extracellular domain contains one Ig-like domain and a fibronectin type III motif. The cytoplasmic domain shares homology with the intracellular domains of IL-17 receptor family members and shows one TIR (Toll/IL-1 Receptor) domain and a putative TRAF6-binding motif (2). Natural IL-17 RD has been shown to form homomultimeric complexes (3). Unlike the alternate splice form of IL-17 RD that has a restricted pattern of expression, the full-length IL-17 RD isoform is expressed in most adult tissues and during embryonic development (3, 5). Functionally, IL-17 RD has been shown to be an inhibitor of FGF signaling. The molecule’s extracellular domain does not seem to be involved. There is an interaction between the intracellular domains of FGFR1/2 and IL-17 RD that blocks ERK dissociation from MEK, thereby interfering with downstream ERK activation of nuclear Elk-1 (8). IL-17 RD has also been reported to interact with TAK1 and induce JNK activation and apoptosis (9). Ligands that interact with the extracellular domain of IL-17 RD have not been identified.

  1. Furthauer, M. et al. (2002) Nat. Cell Biol. 4:170.
  2. Xiong, S. et al. (2003) J. Biol. Chem. 278:50273.
  3. Yang, R-B. et al. (2003) J. Biol. Chem. 278:33232.
  4. Preger, E. et al. (2003) Proc. Natl. Acad. Sci. USA 101:1229.
  5. Lin, W. et al. (2002) Mech. Dev. 113:163.
  6. Tsang, M. et al. (2002) Nat. Cell Biol. 4:165.
  7. Kovalenko, D. et al. (2003) J. Biol. Chem. 278:14087.
  8. Torii, S. et al. (2004) Dev. Cell 7:33.
  9. Yang, X. et al. (2004) J. Biol. Chem. 279:38099.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-17 RD/SEF Antibody (AF2275)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for IL-17 RD/SEF Antibody (AF2275) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2275:
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/06/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22975329
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22975329

Details

Detection NotesSee PMID 22975329

Comments

CommentsPublished: See PMID 22975329

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Anonymous
01/06/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol IL17RD
Entrez
Uniprot