IL-15 R alpha Antibody (JM7A4)

Images

 
Flow Cytometry: IL-15 R alpha Antibody (JM7A4) [NBP1-43238] - Staining of adherent human PBMCs stimulated with LPS and IFN gamma for 24 hours with 0.25 ug of Mouse IgG2b K Isotype Control Purified (NBP1-43317) (open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP
Clone
JM7A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

IL-15 R alpha Antibody (JM7A4) Summary

Immunogen
The immunogen for this antibody was IL15Ra.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
IL15RA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry <0.5 mg/10^6 cells in 100 ul
  • Immunoprecipitation 1:10-1:500
Application Notes
This JM7A4 antibody has been tested by flow cytometric analysis of LPS and IFN-γ-stimulated PBMCs. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Publications
Read Publications using NBP1-43238.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for IL-15 R alpha Antibody (JM7A4)

  • CD215 antigen
  • CD215
  • IL-15 R alpha
  • IL-15 receptor subunit alpha
  • IL15R alpha
  • IL15RA
  • IL-15Ra
  • IL-15R-alpha
  • interleukin 15 receptor, alpha
  • interleukin-15 receptor subunit alpha
  • MGC104179

Background

The JM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15Ralpha). IL-15 and IL-2 are cytokines with overlapping, but distinct, biologic effects. Their receptors share 2 subunits, the IL2R-beta and -gamma chains, which are essential for signal transduction. The IL-15R-alpha receptor is structurally related to IL-2R-alpha. However, the IL-15Ralpha alone binds IL-15 with a 1,000-fold higher affinity than that seen with IL-2R-alpha and IL-2. In the human, 3 differentially spliced human IL15R-alpha variants that are all capable of high affinity binding of IL-15 have been isolated. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, is dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high affinity binding. At high concentrations, IL-15, like IL-2, is able to signal through a complex of IL2R-beta and IL2R-gamma in the absence of the alpha subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-15 R alpha Antibody (NBP1-43238)(4)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL15RA
Entrez
Uniprot