Flow Cytometry: IL-15 R alpha Antibody (JM7A4) [NBP1-43238] - Staining of adherent human PBMCs stimulated with LPS and IFN gamma for 24 hours with 0.25 ug of Mouse IgG2b K Isotype Control Purified (NBP1-43317) (open ...read more
Aqueous buffer, may contain carrier protein/stabilizer (proprietary).
0.09% Sodium Azide
Protein A or G purified
This JM7A4 antibody has been tested by flow cytometric analysis of LPS and IFN-γ-stimulated PBMCs. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Alternate Names for IL-15 R alpha Antibody (JM7A4)
IL-15 receptor subunit alpha
interleukin 15 receptor, alpha
interleukin-15 receptor subunit alpha
The JM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15Ralpha). IL-15 and IL-2 are cytokines with overlapping, but distinct, biologic effects. Their receptors share 2 subunits, the IL2R-beta and -gamma chains, which are essential for signal transduction. The IL-15R-alpha receptor is structurally related to IL-2R-alpha. However, the IL-15Ralpha alone binds IL-15 with a 1,000-fold higher affinity than that seen with IL-2R-alpha and IL-2. In the human, 3 differentially spliced human IL15R-alpha variants that are all capable of high affinity binding of IL-15 have been isolated. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, is dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high affinity binding. At high concentrations, IL-15, like IL-2, is able to signal through a complex of IL2R-beta and IL2R-gamma in the absence of the alpha subunit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.