IL‑13 R alpha 2 was detected in immersion fixed mouse splenocytes using Mouse IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
IL‑13 R alpha 2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL overnight at 4 °C. ...read more
Mouse myeloma cell line NS0-derived recombinant mouse IL‑13 R alpha 2 Leu22-Lys334 Accession # O88786
Detects mouse IL-13 R alpha 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) IL-13 R alpha 2 is observed and less than 1% cross-reactivity with rhIL-5R alpha, rhIL-5R beta, rhIL-9 R, and rhIL-4 R is observed.
Immunogen affinity purified
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for IL-13 R alpha 2 Antibody [Unconjugated]
cancer/testis antigen 19
IL-13 R alpha 2
IL-13 receptor subunit alpha-2
IL13R alpha 2
IL-13R subunit alpha-2
interleukin 13 binding protein
interleukin 13 receptor alpha 2 chain
interleukin 13 receptor, alpha 2
interleukin-13 receptor subunit alpha-2
Two type1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
The mouse IL-13 R alpha 2 cDNA encodes a 383 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 313 residue extracellular domain, a 22 aa residue transmembrane region, and a 27 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 2 share 59% aa sequence identity. The extracellular domain of IL-13 R alpha 2 is also closely related to that of IL-13 R alpha 1. However, the cytoplasmic domain of IL-13 R alpha 2 lacks the box 1 and box 2 signaling motif and is much shorter than that of IL-13 R alpha 1, suggesting that the two receptors are functionally distinct. IL-13 R alpha 1 has been shown to combine with IL-4 R alpha to form a high-affinity receptor complex capable of transducing both an IL-4-dependent and an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated. The amino-terminal 27 amino acid residues of mouse IL-13 R alpha 2 are identical to that of a soluble mouse IL-13 binding protein purified from mouse serum and urine. Recombinant mouse IL-13 R alpha /Fc chimera has been shown to bind IL-13 with high affinity and is a potent IL-13 antagonist.
Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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