IL-13 R alpha 1 Antibody

Images

 
Recombinant Human IL‑13 (Catalog # 213‑ILB) stimulates prolif­eration in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑13 ...read more
IL‑13 R alpha 1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL‑13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) at 15 µg/mL overnight ...read more
Human whole blood granulocytes were stained with Goat Anti-Human IL‑13 R alpha 1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF152, filled histogram) or control antibody (Catalog # AB-108-C, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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IL-13 R alpha 1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 1
Ala27-Thr343 (Thr130Ile)
Accession # Q5JSL4
Specificity
Detects human IL‑13 R alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinat human (rh) IL-13 R alpha 2, recombinant mouse IL-13 R alpha 1, rhIL-5 R alpha, rhIL-5 R beta, rhIL-4 R, and rhIL-9 R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL13RA1
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Neutralization 10-30 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF152 in the following applications:

Publications
Read Publications using
AF152 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-13 R alpha 1 Antibody

  • bB128O4.2.1 (interleukin 13 receptor, alpha 1)
  • Cancer/testis antigen 19
  • CD213a1 antigen
  • CD213a1
  • CT19
  • IL-13 R alpha 1
  • IL13 receptor alpha-1 chain
  • IL-13 receptor subunit alpha-1
  • IL13R alpha 1
  • IL-13R subunit alpha-1
  • IL13R
  • IL13RA
  • IL-13Ra
  • IL13RA1
  • IL-13Ra1
  • IL-13R-alpha-1
  • interleukin 13 receptor, alpha 1
  • interleukin-13 receptor subunit alpha-1
  • NR4

Background

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2. The human IL-13 R alpha 1 was originally cloned based on sequence homology to the mouse IL-13 R alpha 1. The IL‑13 R alpha 1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 1 share 76% aa sequence identity. The extracellular domain of IL‑13 R alpha 1 is also closely related to that of IL-13 R alpha 2. IL-13 R alpha 1 has been shown to combine with the IL-4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated.

  1. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
  2. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
  3. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  4. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
  5. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-13 R alpha 1 Antibody (AF152)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow, IHC Paraffin-embedded, Neut.


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Flow
(2)
IHC Paraffin-embedded
(3)
Neut
(2)
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Human
(7)
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Showing Publications 1 - 7 of 7.
Publications using AF152 Applications Species
Xu J, Singhera G, Dorscheid D Expression of surfactant protein D in airways of asthmatics and interleukin-13 modulation of surfactant protein D in human models of airway epithelium. Respir Res, 2015;16(0):26. 2015 [PMID: 25848896] (Neut,, Human) Neut Human
Reece P, Gauvreau G, Sehmi R, Denburg J IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells. PLoS ONE, 2014;9(6):e100734. 2014 [PMID: 24971469] (Neut,, Human) Neut Human
Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro. PLoS ONE, 2014;9(5):e96925. 2014 [PMID: 24819779] (Flow,, Human) Flow Human
Lama VN, Harada H, Badri LN, Flint A, Hogaboam CM, McKenzie A, Martinez FJ, Toews GB, Moore BB, Pinsky DJ Obligatory role for interleukin-13 in obstructive lesion development in airway allografts. Am. J. Pathol., 2006;169(1):47-60. 2006 [PMID: 16816360] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis. Int. J. Oncol., 2004;25(3):605-13. 2004 [PMID: 15289861] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Kajiwara K, Shinazawa M, Morishima H, Yanagihara Y Differential effect of IL-4 and IL-13 on the expression of recombination-activating genes in mature B cells from human peripheral blood. Cell. Immunol., 2004;227(2):121-8. 2004 [PMID: 15135294] (Flow,, Human) Flow Human
Zhou Y, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome. Am. J. Pathol., 2002;160(4):1405-23. 2002 [PMID: 11943725] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human

Review for IL-13 R alpha 1 Antibody (AF152) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF152:
Filter by Applications
Flow
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Human 01/05/2015
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Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 22092872
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22092872

Details

Detection NotesSee PMID 22092872

Comments

CommentsPublished See PMID 22092872

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Recent Reviews

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Anonymous
01/05/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol IL13RA1
Entrez
Uniprot