Recombinant Mouse IL-12 Protein

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Product Details

Summary
Product Discontinued
View other related IL-12 Peptides and Proteins

Order Details


    • Catalog Number
      NBP1-42917
    • Availability
      Product Discontinued

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Recombinant Mouse IL-12 Protein Summary

Description
A full-length protein corresponding to IL12A.
Preparation
Method
Insect cells infected with baculovirus: mouse p40, amino acids met 1-ser 335, (accession # NM_008352) was co-expressed with mouse p35, amino acids met 1-ala 215, (accession # NM_008351).
Details of Functionality
This protein has been tested in bioassay for induction of IFN-gamma; production, yielding an ED50 of less than 0.3 ng/ml, corresponding to a specific activity of approximately 3.0x10^6 Units/mg.
Protein/Peptide Type
Full Length Recombinant Protein
Gene
IL12A

Applications/Dilutions

Application Notes
This recombinant mouse IL-12 has been tested by sandwich ELISA using the mouse IL-12 p40 ELISA (C15.6/C17.8) and the mouse IL-12 p70 ELISA (C18.2/C17.8).
Publications
Read Publication using NBP1-42917.

Reactivity Notes

This protein was made to the mouse sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile liquid; 10 mM NaH2PO4, pH 6.0, 300 mM NaCl, with no carrier or stabilizer. 0.22 uM filtered.
Preservative
0.09% Sodium Azide

Alternate Names for Recombinant Mouse IL-12 Protein

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. sciences recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. It is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for IL-12 Protein (NBP1-42917)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP1-42917 Applications Species
Shukla S, Wen AM, Commandeur U. Presentation of HER2 epitopes using a filamentous plant virus-based vaccination platform. The Royal Society of Chemistry. 2014 Aug 04

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Bioinformatics

Gene Symbol IL12A
Entrez