Recombinant Human IL-12 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IA
Concentration
0.5 mg/ml

Order Details

Recombinant Human IL-12 Protein Summary

Description
Recombinant protein corresponding to amino acids Met1-Ser 328 of human IL-12. Accession #: NM_002187.

Source: : Insect cells infected with baculovirus

Details of Functionality
This IL-12 p70 protein has been tested by bioassay, yielding an ED50 of 100 pg/ml, corresponding to a specific activity of approximately 1x10^7 Units/mg.
Source
Sf 21 (baculovirus)
Protein/Peptide Type
Biologically Active Protein
Gene
IL12A
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 0.01 ng/ug cytokine, as determined by the LAL assay.

Applications/Dilutions

Application Notes
This recombinant human IL-12 p70 has been tested by sandwich ELISA using the human IL-12 p70 ELISA (B-T21/C8.6).
Theoretical MW
57.23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline, pH 6.0
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human IL-12 Protein

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-gamma) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-gamma and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol IL12A
Entrez