IL-10 Antibody (JES3-12G8)

Product Discontinued

IL-10 Antibody (JES3-12G8) Summary

• Immunogen: Human IL-10 expressed by COS cells
• Localization: Secreted
• Specificity: NB100-63262 is specific for human Interleukin-10 (IL-10). IL-10 is expressed by activated CD8+ peripheral blood T-cells, activated T-helper CD4+ T cell clones mast cells, B cell lymphomas and LPS activated monocytes. This clone is reported to neutralise the effects of IL-10.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL10
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:100-1:250
• Application Notes: For Flow Cytometry: Membrane permeabilisation is required for this application. For IHC: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; sodium citrate buffer pH 6.0 is recommended for this purpose. Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Although not tested this antibody may be useful in IHC-F.
• Control:

  IL-10 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: BBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for IL-10 Antibody (JES3-12G8)

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 160 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL10
• Entrez:
  • 3586(Human)
• Uniprot:
  • P22301()