IL-1 RII Antibody

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-77884
    • Availability
      Product Discontinued

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IL-1 RII Antibody Summary

Immunogen
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1R type II.
Specificity
ThisisanIgGpreparationofwholerabbitserumpurifiedbyProteinAchromatography.TheantibodyisdirectedagainsttheextracellulardomainofthehumanIL-1RtypeII,butprecipitatestheintactreceptorexpressedonhumancells.
Clonality
Polyclonal
Host
Rabbit
Gene
IL1R2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:1000-1:5000
Application Notes
This IgG fraction antibody of anti-Human IL-1 Receptor Type II has been tested for use in ELISA, radioimmunoassays, flow cytometry and immunoprecipitation. Reactivity in other immunoassays is unknown. IL-1RII has been termed p68 or CD121 in some laboratories. IL-1RII has been shown - bind IL-1, IL-1a and IL-1b.This product has been assayed for the ability - immunoprecipitate antigen. A dilution range of 1:400 - 1:800 is suggested for this immunoassay. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful - reduce background. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] and TMB as a substrate. A working dilution range of 1:200 - 1:400 is suggested for this application. This product has been assayed by ELISA against soluble IL-1RII using HRP Conjugated Anti-Rabbit IgG [H&L] and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:1,000 - 1:2000 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8,000 is suggested for this immunoassay.

Reactivity Notes

Reactive in human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for IL-1 RII Antibody

  • beta
  • CD121b antigen
  • CD121b
  • IL-1 R beta
  • IL-1 RII
  • IL1R2
  • IL1RBCD121 antigen-like family member B
  • IL-1R-beta
  • IL1RII
  • IL-1RII
  • IL-1RT2
  • IL-1RT-2
  • interleukin 1 receptor, type II
  • Interleukin-1 receptor beta
  • MGC47725

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL1R2
Entrez