Recombinant Mouse IL-1 beta/IL-1F2 Protein

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Summary
Product Discontinued
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    • Catalog Number
      NBP1-99956
    • Availability
      Product Discontinued

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Recombinant Mouse IL-1 beta/IL-1F2 Protein Summary

Description
A recombinant protein corresponding to 153 amino acids of Mouse IL-1 beta/IL-1F2
Preparation
Method
>98% SDS-PAGE
Details of Functionality
Biological Activity: The ED50 determined by the dose-dependent stimulation of murine D10S cells is <0.002 ng/ml, corresponding to a specific activity of >5 x 10^8 units/mg.
Results: <0.002 ng/ml
Protein/Peptide Type
Recombinant Protein
Gene
IL1B
Purity
>90% SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
From 5 mM Tris, pH 8.0.
Concentration
Lyoph
Purity
>90% SDS-PAGE
Reconstitution Instructions
Reconstitute with water to a final concentration of 0.1 - 1.0 mg/ml.

Notes

After reconstituted, dilute further with other aqueous solutions, and stored at 4C for 1 week or -20C for future usage.

Alternate Names for Recombinant Mouse IL-1 beta/IL-1F2 Protein

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1Beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1Beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1Beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant murine IL-1Beta is a 17.5 kDa protein containing 153 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Blogs on IL-1 beta/IL-1F2.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Bioinformatics

Gene Symbol IL1B
Entrez