IL-1 beta/IL-1F2 Antibody [HRP]

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Western Blot: IL-1 beta/IL-1F2 Antibody [HRP] [NBP1-42753] - IL1 beta Antibody [HRP] [NBP1-42753] - This antibody is primarily directed against mature, 17,000 MW human IL-1beta

Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP1-42753
    • Availability
      Product Discontinued

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IL-1 beta/IL-1F2 Antibody [HRP] Summary

Description
This antibody was heated to 56 C for 30 minutes.
Immunogen
This antibody was prepared by repeated immunizations with recombinant human IL-1 beta produced in E.coli. The MW of the recombinant 153 aa IL-1 beta was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1 beta converting enzyme (ICE, capase-1).
Specificity
This antibody is primarily directed against mature, 17,000 MW human IL-1 beta.
Clonality
Polyclonal
Host
Rabbit
Gene
IL1B
Purity
Ion exchange chromatography
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 1:500-1:2500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody recognizes the 17,000 MW mature IL-1Beta. For immunoblots, typically, IL-1Beta is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells. PBMC are stimulated for 24 hours with 1% serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG - reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation - stain intracellular IL-1Beta. This antibody is also useful for neutralization of human and primate IL-1Beta activity in bioassays. It does not neutralize the biological activity IL-1alpha. It does not neutralize the biological activity of murine, rat or rabbit IL-1Beta. For neutralization, it is recommended - incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used f. Caution should be exhibited as the F domain of the rabbit IgG molecule may interact with cells non-specifically.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water to a final concentration of 1.0 mg/ml.

Alternate Names for IL-1 beta/IL-1F2 Antibody [HRP]

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 beta (also known as Interleukin-1 beta, IL-1beta and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1beta is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics Tool for IL-1 beta/IL-1F2 Antibody (NBP1-42753)

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Diseases for IL-1 beta/IL-1F2 Antibody (NBP1-42753)

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Blogs on IL-1 beta/IL-1F2.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Bioinformatics

Gene Symbol IL1B
Entrez