IL-1 beta/IL-1F2 Antibody

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Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42751] - Detection of 17,000 MW human IL-1Beta and is useful in determining its presence in various assays.
Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42751] - Analysis using HRP conjugate of NBP1-42751. This antibody is primarily directed against mature, 17,000 MW human IL-1beta

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42751
    • Availability
      Product Discontinued

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IL-1 beta/IL-1F2 Antibody Summary

Description
This is an IgG preparation of whole rabbit serum purified by DEAE fractionation.
Immunogen
This antibody was prepared by repeated immunizations with recombinant human IL-1 beta produced in E.coli. The MW of the recombinant 153 aa IL-1 beta was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1 beta converting enzyme (ICE, capase-1).
Specificity
This antibody is primarily directed against mature, 17,000 MW human IL-1 beta.
Clonality
Polyclonal
Host
Rabbit
Gene
IL1B
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:1000-1:5000
Application Notes
Anti-Human IL-1 beta has been tested for use in neutralizations, ELISA, radioimmunoassays, flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17 kDa mature IL-1 beta.
Publications
Read Publication using
NBP1-42751 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
No Preservative
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml distilled water.

Alternate Names for IL-1 beta/IL-1F2 Antibody

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 beta (also known as Interleukin-1 beta, IL-1 beta and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1 beta is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1 beta/IL-1F2 Antibody (NBP1-42751)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional IL-1 beta/IL-1F2 Products

Bioinformatics Tool for IL-1 beta/IL-1F2 Antibody (NBP1-42751)

Discover related pathways, diseases and genes to IL-1 beta/IL-1F2 Antibody (NBP1-42751). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for IL-1 beta/IL-1F2 Antibody (NBP1-42751)

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Pathways for IL-1 beta/IL-1F2 Antibody (NBP1-42751)

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PTMs for IL-1 beta/IL-1F2 Antibody (NBP1-42751)

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Research Areas for IL-1 beta/IL-1F2 Antibody (NBP1-42751)

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Blogs on IL-1 beta/IL-1F2.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Bioinformatics

Gene Symbol IL1B
Entrez