IL-1 beta/IL-1F2 Antibody (3A6)

Images

 
Western Blot: IL-1 beta/IL-1F2 Antibody (3A6) [NBP2-22184] - Western blot analysis using IL1 beta mAb against human IL1B (AA: 126-261) recombinant protein. (Expected MW is 41 kDa)
Immunocytochemistry/ Immunofluorescence: IL-1 beta/IL-1F2 Antibody (3A6) [NBP2-22184] - Immunofluorescence analysis of HepG2 cells using IL1 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments ...read more
Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (3A6) [NBP2-22184] - Immunohistochemical analysis of paraffin-embedded muscle tissues using IL1 beta mouse mAb with DAB staining.
Western Blot: IL-1 beta/IL-1F2 Antibody (3A6) [NBP2-22184] - Western blot analysis using IL1 beta mAb against HEK293 (1) and IL1B-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (3A6) [NBP2-22184] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using IL1 beta mouse mAb with DAB staining.
ELISA: IL-1 beta/IL-1F2 Antibody (3A6) [NBP2-22184] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-22184
    • Availability
      Product Discontinued

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IL-1 beta/IL-1F2 Antibody (3A6) Summary

Immunogen
Purified recombinant fragment of human IL1 beta expressed in E. Coli.
Localization
Cytoplasm
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL1B
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for IL-1 beta/IL-1F2 Antibody (3A6)

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional IL-1 beta/IL-1F2 Products

Bioinformatics Tool for IL-1 beta/IL-1F2 Antibody (NBP2-22184)

Discover related pathways, diseases and genes to IL-1 beta/IL-1F2 Antibody (NBP2-22184). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for IL-1 beta/IL-1F2 Antibody (NBP2-22184)

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Pathways for IL-1 beta/IL-1F2 Antibody (NBP2-22184)

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PTMs for IL-1 beta/IL-1F2 Antibody (NBP2-22184)

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Research Areas for IL-1 beta/IL-1F2 Antibody (NBP2-22184)

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Blogs on IL-1 beta/IL-1F2.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Bioinformatics

Gene Symbol IL1B
Entrez