Recombinant Mouse IL-1 alpha/IL-1F1 Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43231
    • Availability
      Product Discontinued

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Recombinant Mouse IL-1 alpha/IL-1F1 Protein Summary

Description
A Partial Recombinant Proteins to IL-1 alpha/IL-1F1.
Preparation
Method
E. coli expressed amino acids Ser 115-Ser 270 of mouse IL-1a (accession # BC003727).
Details of Functionality
Measured by mouse D10S cell proliferation assay. The ED50 is 0.01 ng/ml, corresponding to a specific activity of 1.0 x 108 U/mg.
Protein/Peptide Type
Partial Recombinant Protein
Gene
IL1A
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Application Notes
This reagent has been tested by sandwich ELISA using the anti-mouse IL-1α ELISA pair (ALF-161/biotin-polyclonal).

Reactivity Notes

This protein was made to the mouse sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile liquid; 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, 1.0 BSA. 0.22 um filtered.
Preservative
0.09% Sodium Azide

Notes

Purity: Greater than 98%, as determined by SDS-PAGE

Alternate Names for Recombinant Mouse IL-1 alpha/IL-1F1 Protein

  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

Mouse IL-1alpha, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is an ~17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1alpha is mostly cell-associated, with 23% amino acid homology with IL-1beta. The immune regulatory role of IL-1alpha is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IL1A
Entrez