Recombinant Human IL-1 alpha/IL-1F1 Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42929
    • Availability
      Product Discontinued

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Recombinant Human IL-1 alpha/IL-1F1 Protein Summary

Description
A partial protein corresponding to IL1A.
Preparation
Method
E. coli.
Details of Functionality
Measured by mouse D10S cell proliferation assay. The ED50 of 20 pg/ml, corresponding to a specific activity of approximately 5x10^7Units/mg.
Protein/Peptide Type
Partial Recombinant Protein
Gene
IL1A
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Application Notes
The recombinant human IL-1α has been tested as the standard in a human IL-1α sandwich ELISA using the human IL-1α ELISA pair (CRM8/CRM6).

Reactivity Notes

This protein was made to the human sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile liquid; 20 mM tris, 0.2 M NaCl, 1 mM DTT, pH 8.0 with no carriers or stabilizers. 0.22 um filtered.
Preservative
0.09% Sodium Azide

Notes

Purity: Greater than 98%, as determined by SDS-PAGE

Alternate Names for Recombinant Human IL-1 alpha/IL-1F1 Protein

  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

Human IL-1alpha, also called lymphocyte activating factor (LAF), endogenous pyrogen (EP), leucocyte endogenous mediator (LEM), and mononuclear cell factor (MCF), is produced by a wide variety of cells, including macrophages, dendritic cells, T cells and B cells. IL-1alpha is mostly cell associated and has only 20% amino acid homology with IL-1beta. The immune regulatory role of IL-1alpha is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IL1A
Entrez