IL-1 alpha/IL-1F1 ELISA Kit


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Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)

Order Details

IL-1 alpha/IL-1F1 ELISA Kit Summary

Standard Curve Range
109 - 7,000 pg/mL
30 pg/mL
Kit Type

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.


Application Notes
The human IL-1RA ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-1RA

Kit Components

  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal polyclonal antibody to human IL-1RA
  2. Biotin-Conjugate anti-human IL-1RA polyclonal antibody
  3. Streptavidin-HRP
  4. Human IL-1RA Standard lyophilized, 14 ng/ml upon reconstitution
  5. Sample Diluent
  6. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  7. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  8. Substrate Solution (tetramethyl-benzidine)
  9. Stop Solution (1M Phosphoric acid)
  10. Adhesive Films

Reactivity Notes



Colored dyes for optional use with this kit are available and will be provided free of charge upon request. Please refer to catalog number NBP2-41404.

Alternate Names for IL-1 alpha/IL-1F1 ELISA Kit

  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha


Interleukin 1 receptor antagonist is a protein related to the IL-1 family based on its similarities in amino acid sequence to both IL-1 beta and IL-1 alpha, similarities in gene structure, and collective gene localization. The human gene contains four exons and maps to chromosome 2q13-14. IL-1 RA binds to the IL-1 receptor and blocks IL-1 action through competitive inhibition. In contrast to IL-1, the binding of IL-1RA does not initiate signaling events. The secretion of IL-1 RA is mediated by a classical signal sequence while neither IL-1alpha nor IL-1beta possess such a sequence. The lack of homology between IL-1 RA and IL-1 alpha or IL-1 beta near the N terminus may provide an explanation for the biosynthetic and functional differences between IL-1 RA and the two IL-1 proteins. The production of IL-1RA is stimulated by many substances including adherent IgG, cytokines, and bacterial or viral components. IL-1RA is an important natural anti-inflammatory protein that when missing or just available in inadequate amounts plays a key role in several pathological conditions such as rheumatoid arthritis, colitis, osteomyelitis, periostitis and pustulosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Gene Symbol IL1A

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