IL-1 alpha/IL-1F1 Antibody

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Western Blot: IL1 alpha Antibody [NBP1-45645] - Western blot analysis of human IL-1a using recombinant human hIL-1a Lane 1: 10 ng Lane 2: 50 ng

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-45645
    • Availability
      Product Discontinued

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IL-1 alpha/IL-1F1 Antibody Summary

Immunogen
E. coli expressed human IL-1α.
Clonality
Polyclonal
Host
Rabbit
Gene
IL1A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Neutralization
Application Notes
Western blot analysis (0.5-2 ug/ml). Per researcher's feedback, it can also be used in neutralization (5-10 ug/ml). However, the optimal conditions should be determined individually. Recombinant human IL-1alpha can be used as a positive control.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for IL-1 alpha/IL-1F1 Antibody

  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

The ALF-161 antibody reacts with mouse interleukin-1alpha (IL-1alpha). Mouse IL-1alpha, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1alpha is mostly cell-associated, with 23% amino acid homology with IL-1beta. The immune regulatory role of IL-1alpha is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional IL-1 alpha/IL-1F1 Products

Bioinformatics Tool for IL-1 alpha/IL-1F1 Antibody (NBP1-45645)

Discover related pathways, diseases and genes to IL-1 alpha/IL-1F1 Antibody (NBP1-45645). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for IL-1 alpha/IL-1F1 Antibody (NBP1-45645)

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PTMs for IL-1 alpha/IL-1F1 Antibody (NBP1-45645)

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Bioinformatics

Gene Symbol IL1A
Entrez
Uniprot