IL-1 alpha/IL-1F1 Antibody (ALF-161)


Flow Cytometry: IL-1 alpha/IL-1F1 Antibody (ALF-161) [NBP1-43247] - Analysis using the FITC conjugate of NBP1-43247. Mouse thiogylcolate-induced peritoneal exudate cells (PECs) were stimulated with LPS in the presence more
ELISA: IL-1 alpha/IL-1F1 Antibody (ALF-161) [NBP1-43247] - Mouse splenocytes (500,000 cells per well) were activated with LPS (1 ug/ml) for 48 hrs in mouse IL-1 alpha ELISPOT assay. Control well is medium alone.

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IL-1 alpha/IL-1F1 Antibody (ALF-161) Summary

The immunogen for this antibody was IL1a.
Armenian Hamster
Protein A or G purified
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  • ELISA 1:100-1:2000
  • Flow Cytometry
Application Notes
The ALF-161 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-1 alpha (IL-1α) in combination with the biotin anti-mouse Interleukin-1 alpha (IL-1a) polyclonal antibody for detection and recombinant mouse IL-1a as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000pg/ml - 8pg/ml should be included in each ELISA plate.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous, may contain carrier protein/stabilizer.
0.09% Sodium Azide
Protein A or G purified

Alternate Names for IL-1 alpha/IL-1F1 Antibody (ALF-161)

  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha


The ALF-161 antibody reacts with mouse interleukin-1alpha (IL-1alpha). Mouse IL-1alpha, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1alpha is mostly cell-associated, with 23% amino acid homology with IL-1beta. The immune regulatory role of IL-1alpha is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol IL1A