IL-1 alpha/IL-1F1 Antibody (ALF-161)

Images

 
Flow Cytometry: IL-1 alpha/IL-1F1 Antibody (ALF-161) [NBP1-43247] - Analysis using the FITC conjugate of NBP1-43247. Mouse thiogylcolate-induced peritoneal exudate cells (PECs) were stimulated with LPS in the presence ...read more
ELISA: IL-1 alpha/IL-1F1 Antibody (ALF-161) [NBP1-43247] - Mouse splenocytes (500,000 cells per well) were activated with LPS (1 ug/ml) for 48 hrs in mouse IL-1 alpha ELISPOT assay. Control well is medium alone.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43247
    • Availability
      Product Discontinued

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IL-1 alpha/IL-1F1 Antibody (ALF-161) Summary

Immunogen
The immunogen for this antibody was IL1a.
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
IL1A
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry
Application Notes
The ALF-161 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-1 alpha (IL-1α) in combination with the biotin anti-mouse Interleukin-1 alpha (IL-1a) polyclonal antibody for detection and recombinant mouse IL-1a as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000pg/ml - 8pg/ml should be included in each ELISA plate.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for IL-1 alpha/IL-1F1 Antibody (ALF-161)

  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

The ALF-161 antibody reacts with mouse interleukin-1alpha (IL-1alpha). Mouse IL-1alpha, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1alpha is mostly cell-associated, with 23% amino acid homology with IL-1beta. The immune regulatory role of IL-1alpha is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Diseases for IL-1 alpha/IL-1F1 Antibody (NBP1-43247)

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Bioinformatics

Gene Symbol IL1A
Entrez