IKK beta Antibody

Images

 
Western Blot: IKK beta Antibody [NBP1-76935] - Analysis of IKK b in Jurkat whole cell lysate with anti-IKK b (C3) using NBP1-76935 at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: IKK beta Antibody [NBP1-76935] - Immunofluorescence of IKK beta in Hela cells with IKK beta antibody at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: IKK beta Antibody [NBP1-76935] - Immunocytochemical analysis of IKK b in formalin-fixed HeLa cells using NBP1-76935 at 10 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

IKK beta Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IKK beta (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid. The immunogen is located within amino acids 650 - 700 of IKK beta.
Specificity
This polyclonal antibody has no cross response to IKKa or IKKy.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IKBKB
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
IKK beta antibody can be used for detection of IKK beta by Western blot at 1 - 2 mg/ml. A 87 kDa band should be detected. This polyclonal antibody has no cross response to IKKa or IKKy.
Theoretical MW
87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
IKK beta Peptide (NBP1-76935PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IKK beta Antibody

  • EC 2.7.11
  • EC 2.7.11.10
  • I-kappa-B kinase 2
  • I-kappa-B-kinase beta
  • IkBKB
  • IKK beta
  • IKK2
  • IKK2FLJ38368
  • IKK-B
  • IKK-betaFLJ36218
  • IKKBFLJ40509
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
  • inhibitor of nuclear factor kappa-B kinase subunit beta
  • MGC131801
  • NFKBIKB
  • NFKBIKBFLJ33771
  • Nuclear factor NF-kappa-B inhibitor kinase beta

Background

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha, and bacteria product LPS. NF-kappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. The long-sought IkappaB kinase (IKK), which phosphorylates IkappaB, and mediates IkappaB degradation and NF-kappaB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKalpha and IKKbeta). IKKalpha and IKKbeta interact with each other and both are essential for NF-kappaB activation. IKKbeta phosphorylates both IkappaB-alpha and IkappaB-beta. IKKbeta is expressed in variety of human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on IKK beta.

Regulating Immune Response Pathways with IKK beta
IKK beta, also known as IKK2, activates the NFkB complex by phosphorylating the NFkB inhibitor, IkBa. Several transcript variants, some protein-coding and some not, have been found for IKKB. The Nuclear Factor-kappa B (NF-kB) family of transcription f...  Read full blog post.

IKK alpha: Roles in Development, B-cell Survival and ESC Differentiation
Inhibitor of nuclear factor kappa-B kinase subunit alpha (IKK1 alpha) is a serine/threonine kinase that forms a complex with IKK beta and NEMO. It plays an essential role in embryonic skin development. Mice with low levels of IKKa show an increase in...  Read full blog post.

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Bioinformatics

Gene Symbol IKBKB
Entrez
Uniprot