IkB-beta [p Ser23] Antibody

Images

 
Western Blot: IkB-beta [p Ser23] Antibody [NB100-92564] - Analysis of p-IkB-beta (S23) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
Immunohistochemistry-Paraffin: IkB-beta [p Ser23] Antibody [NB100-92564] - Paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead ...read more
Western Blot: IkB beta [p Ser23] Antibody [NB100-92564] - Western blot (WB) analysis of p-I kappa B-alpha (S23) antibody in extracts from HeLa cells.
Western Blot: IkB beta [p Ser23] Antibody [NB100-92564] Extracts from THP1 cells.
Immunohistochemistry: IkB beta [p Ser23] Antibody [NB100-92564] - Immunohistochemistry (IHC) analyzes of p-I kappa B-alpha (S23) antibody in paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92564
    • Availability
      Product Discontinued

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IkB-beta [p Ser23] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human IkB-beta around the phosphorylation site of Serine 23.
Modification
p Ser23
Clonality
Polyclonal
Host
Rabbit
Gene
NFKBIB
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Western blot (WB) analysis of p-IkB-Beta (S23) pAb in extracts from THP1 cells.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IkB-beta [p Ser23] Antibody

  • beta
  • IkappaBbeta
  • I-kappa-B-beta
  • ikB-B
  • IkBbeta
  • IkB-beta
  • IKBBikB-beta
  • NF-kappa-B inhibitor beta
  • NF-kappa-BIB
  • NFKBIB
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor
  • Thyroid receptor-interacting protein 9
  • TR-interacting protein 9
  • TRIP-9
  • TRIP9ikappaBbeta

Background

Three major forms of IkB-like molecules have been identified and each is characterized by multiple copies of ankyrin repeats. IkB-alpha and IkB-beta appear to be the major regulatory forms of IkB in most cells. These proteins interact with p65 or c-Rel containing forms of NFkB and block nuclear import by masking the nuclear localization sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IkB. Immunoblotting easily detects the hyper-phosphorylated forms of IkB-alpha, but not phosphorylated IkB-beta. Interestingly, IkB-alpha and IkB-beta mediate different NFkB responses. IkB-alpha appears to control more transient activation of NFkB in response to an inducer, while IkB-beta controls a persistent response. Bcl-3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IkB is confirmed by immunoblotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IkB-beta Antibody (NB100-92564). (Showing 1 - 1 of 1 FAQ).

  1. I am very concerned about the purification of this antibody. It is not mentioned that it was cross-adsorbed to the non-phospho peptide and I want to confirm that it was adsorbed. Can you help?
    • In regards to this product, from the information I have it was not cross absorbed against a non-phospho specific peptide, and has only been tested on endogenous samples. We will guarantee it to be specific to the phospho protein.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol NFKBIB
Entrez