IkB-alpha [p Tyr42] Antibody

Images

 
Western Blot: IkB-alpha [p Tyr42] Antibody [NBP1-51433] - Analysis of p-IkB-alpha (Y42) pAb at 1:500 dilution Lane1:Hela cell lysate treated with forskolin (10nM, 72h) Lane2:Raw264.7 cell lysate treated with forskolin ...read more
Immunohistochemistry-Paraffin: IkB-alpha [p Tyr42] Antibody [NBP1-51433] - Analyzes of p-IkB-alpha (Y42) pAb in paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51433
    • Availability
      Product Discontinued

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IkB-alpha [p Tyr42] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human IkB-alpha around the phosphorylation site of Tyrosine 42.
Modification
p Tyr42
Specificity
p-IkappaB-alpha (Y42) pAb detects endogenous levels of IkappaB-alphaprotein only when phosphorylated at Y42.
Clonality
Polyclonal
Host
Rabbit
Gene
NFKBIA
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Immunohistochemistry (IHC) analyzes of p-IkB-alpha (Y42) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IkB-alpha [p Tyr42] Antibody

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • NFKBI
  • NFKBIA
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

On the basis of both functional and structural considerations, members of the IkB family of proteins can be divided into four groups. The first of these groups, IkB-alpah (a 35-37 kDa protein), includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to kB sites, suggesting that the IkB-alpha family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IkB family is represented by a 45 kDa protein designated IkB-beta. The third group of IkB proteins is represented by Ik B-gamma, a 70 kDa protein identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and expressed predominantly in lymphoid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics Tool for IkB-alpha Antibody (NBP1-51433)

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Research Areas for IkB-alpha Antibody (NBP1-51433)

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Blogs on IkB-alpha.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

Inhibitor kappa B-alpha (IkappaB-alpha)
The transcription factor nuclear factor kappa beta (NFkB) is highly regulated by triggers such as stress, free-radicals, UV light, and hypoxia. NFkB is one of the fastest responding transcription factors in humans. The NFKB signaling pathway is ess...  Read full blog post.

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Bioinformatics

Gene Symbol NFKBIA
Entrez