IkB-alpha Antibody (5D1623)

Images

 
Western Blot: IkB-alpha Antibody (5D1623) [NB100-56597] - Detection of cleaved IkBalpha in Jurkat cell lysates. Lane A. NB100-56597 incubated in the presence of the immunizing peptide. Lane B. incubated with the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
5D1623
Clonality
Monoclonal
Host
Mouse
Concentration
0.5 mg/ml

IkB-alpha Antibody (5D1623) Summary

Immunogen
This monoclonal antibody was developed against a synthetic peptide corresponding to the caspase-3 mediated cleavage site of IkBa.
Specificity
IkBa (5D1623)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NFKBIA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml

Alternate Names for IkB-alpha Antibody (5D1623)

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • NFKBI
  • NFKBIA
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. Recent studies have shown that IkBa can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkBa (DN-IkBa). The DN-IkBa is resistant to degradation in response to inducers of NF-kB and act as dominant inhibitory molecule that suppresses NF-kB activity during apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
MAB72261
Species: Hu
Applications: WB, Simple Western, ICC
NBP1-91499
Species: Mu, Bv
Applications: WB, ICC/IF
NB600-225
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-02665
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
MAB8930
Species: Hu
Applications: WB, Simple Western, ICC
AF632
Species: Hu
Applications: WB, AgAct
NBP1-47842
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
NB300-605
Species: Hu, Mu, Rt, Po, Av, Fe, Fi, Rb, Xp
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88650
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01345
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-56509
Species: Hu, Mu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP
NB100-56704
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC-Fr, IHC-P, IP, Flow-IC
NB100-689
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
MAB2055
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
AF2699
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
NBP2-46047
Species: Hu
Applications: WB, IHC
NB100-56597
Species: Hu
Applications: WB
Supplier Logo

Publications for IkB-alpha Antibody (NB100-56597) (0)

There are no publications for IkB-alpha Antibody (NB100-56597).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IkB-alpha Antibody (NB100-56597) (0)

There are no reviews for IkB-alpha Antibody (NB100-56597). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IkB-alpha Antibody (NB100-56597) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56597AF405
Alexa Fluor 488 Labeled NB100-56597AF488
Alexa Fluor 647 Labeled NB100-56597AF647
Alexa Fluor 700 Labeled NB100-56597AF700
Allophycocyanin Labeled NB100-56597APC
Biotin Labeled NB100-56597B
DyLight 350 Labeled NB100-56597UV
DyLight 405 Labeled NB100-56597V
DyLight 405LS Labeled NB100-56597V3
DyLight 488 Labeled NB100-56597G
DyLight 550 Labeled NB100-56597R
DyLight 650 Labeled NB100-56597C
DyLight 680 Labeled NB100-56597FR
DyLight 755 Labeled NB100-56597IR
HRP Labeled NB100-56597H
PE Labeled NB100-56597PE
PerCP Labeled NB100-56597PCP

Additional IkB-alpha Antibody (5D1623) Products

Related Products by Gene

Bioinformatics Tool for IkB-alpha Antibody (NB100-56597)

Discover related pathways, diseases and genes to IkB-alpha Antibody (NB100-56597). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IkB-alpha Antibody (NB100-56597)

Discover more about diseases related to IkB-alpha Antibody (NB100-56597).
 

Pathways for IkB-alpha Antibody (NB100-56597)

View related products by pathway.

PTMs for IkB-alpha Antibody (NB100-56597)

Learn more about PTMs related to IkB-alpha Antibody (NB100-56597).
 

Research Areas for IkB-alpha Antibody (NB100-56597)

Find related products by research area.

Blogs on IkB-alpha.

Inhibitor kappa B-alpha (IkappaB-alpha)
The transcription factor nuclear factor kappa beta (NFkB) is highly regulated by triggers such as stress, free-radicals, UV light, and hypoxia. NFkB is one of the fastest responding transcription factors in humans. The NFKB signaling pathway is ess...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol NFKBIA
Entrez
Uniprot

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-56597 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought