IkB-alpha Antibody (5D1623)

Images

 
Western Blot: IkB-alpha Antibody (5D1623) [NB100-56597] - Detection of cleaved IkBalpha in Jurkat cell lysates. Lane A. NB100-56597 incubated in the presence of the immunizing peptide. Lane B. incubated with the ...read more

Product Details

Summary
Product Discontinued
View other related IkB-alpha Primary Antibodies

Order Details


    • Catalog Number
      NB100-56597
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

IkB-alpha Antibody (5D1623) Summary

Immunogen
This monoclonal antibody was developed against a synthetic peptide corresponding to the caspase-3 mediated cleavage site of IkBa.
Specificity
IkBa (5D1623)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NFKBIA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml

Alternate Names for IkB-alpha Antibody (5D1623)

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • NFKBI
  • NFKBIA
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. Recent studies have shown that IkBa can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkBa (DN-IkBa). The DN-IkBa is resistant to degradation in response to inducers of NF-kB and act as dominant inhibitory molecule that suppresses NF-kB activity during apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
MAB72261
Species: Hu
Applications: WB, Simple Western, ICC
NBP1-91499
Species: Mu, Bv
Applications: WB, ICC/IF
NB600-225
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-02665
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
MAB8930
Species: Hu
Applications: WB, Simple Western, ICC
AF632
Species: Hu
Applications: WB, AgAct
NBP1-47842
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
NB300-605
Species: Hu, Mu, Rt, Po, Av, Fe, Fi, Rb, Xp
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88650
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-01345
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-56509
Species: Hu, Mu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NB100-56704
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC-Fr, IHC-P, IP, ChIP, Flow-IC
NB100-689
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
MAB2055
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-ready, ICC, ICFlow
AF2699
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
NBP2-46047
Species: Hu
Applications: WB, IHC
NB100-56597
Species: Hu
Applications: WB
Supplier Logo

Publications for IkB-alpha Antibody (NB100-56597) (0)

There are no publications for IkB-alpha Antibody (NB100-56597).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IkB-alpha Antibody (NB100-56597) (0)

There are no reviews for IkB-alpha Antibody (NB100-56597). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IkB-alpha Antibody (NB100-56597) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IkB-alpha Antibody (5D1623) Products

Related Products by Gene

Bioinformatics Tool for IkB-alpha Antibody (NB100-56597)

Discover related pathways, diseases and genes to IkB-alpha Antibody (NB100-56597). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IkB-alpha Antibody (NB100-56597)

Discover more about diseases related to IkB-alpha Antibody (NB100-56597).
 

Pathways for IkB-alpha Antibody (NB100-56597)

View related products by pathway.

PTMs for IkB-alpha Antibody (NB100-56597)

Learn more about PTMs related to IkB-alpha Antibody (NB100-56597).
 

Research Areas for IkB-alpha Antibody (NB100-56597)

Find related products by research area.

Blogs on IkB-alpha.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

Inhibitor kappa B-alpha (IkappaB-alpha)
The transcription factor nuclear factor kappa beta (NFkB) is highly regulated by triggers such as stress, free-radicals, UV light, and hypoxia. NFkB is one of the fastest responding transcription factors in humans. The NFKB signaling pathway is ess...  Read full blog post.

Contact Information

Product PDFs

Review this Product

Be the first to review our IkB-alpha Antibody (5D1623) and receive a gift card or discount.

Bioinformatics

Gene Symbol NFKBIA
Entrez
Uniprot