IkB-alpha [p Ser32, p Ser36] Antibody (39A1413)

Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NBP3-23385] - Western blot analysis of Jurkat cells untreated (lanes 1 & 3) or treated with TNF alpha (1 nM). The blots were probed with anti-IkB-alpha (lanes 1 & 2) or anti-IkB-alpha (Ser-32/Ser-36) (lanes 3 & 4).

Product Discontinued

IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Clone 39A1413 was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 32 and 36 of human IkBa. This peptide sequence is highly conserved in mouse, rat, dog, cow, and pig IkB-alpha. [UniProt# P25963]
• Modification: p Ser32, p Ser36
• Specificity: The antibody detects a 38 kDa protein on SDS-PAGE immunoblots of Jurkat cells treated with calpain inhibitor (ALLN) followed by TNF alpha, but the antibody does not detect this band in untreated cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFKBIA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.5% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413)

• alpha
• I-kappa-B-alpha
• IkappaBalphaNF-kappa-B inhibitor alpha
• IKBAikB-alpha
• IkBalpha
• IkB-alpha
• MAD3
• MAD-3
• Major histocompatibility complex enhancer-binding protein MAD3
• NFKBI
• NFKBIA
• nuclear factor of kappa light chain gene enhancer in B-cells
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFKBIA