Rabbit anti-Chicken IgY (H+L) Secondary Antibody [HiLyte Fluor 680]

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Product Details

Summary
Reactivity ChSpecies Glossary
Applications Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
HiLyte Fluor 680
Concentration
1 mg/ml

Order Details

Rabbit anti-Chicken IgY (H+L) Secondary Antibody [HiLyte Fluor 680] Summary

Immunogen
Chicken IgY
Specificity
Chicken IgY (H+L)
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Reactivity Notes

Reacts with chicken.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10 mM phosphate, 150 mM NaCl, pH 7.2, with BSA
Preservative
2mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Background

HiLyte Fluor(TM) 680 - IgY conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised IgY function. The fluorescence of HiLyte Fluor(TM) 680 is only slightly red-shifted compared to those of Cy5.5 dyes, resulting in an optimal match to filters designed for Cy5.5 dyes. Fluorescence emission of HiLyte Fluor(TM) 680 is well separated from that of other commonly used red fluorophores, such as TAMRA, R-phycoerythrin and HiLyte Fluor(TM) 647 dyes, making it ideal for three- and four-color labeling. The fluorescence signal of HiLyte Fluor(TM) 680-conjugate can be observed at 699 nm with the excitation at 678 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Additional IgY (H+L) Products

IgY (H+L) NB710-94982

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