Goat anti-Rat IgM Secondary Antibody [DyLight 800]

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Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Antibody [DyLight 800] [NBP1-72980]

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, FLISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 800
Concentration
LYOPH

Goat anti-Rat IgM Secondary Antibody [DyLight 800] Summary

Immunogen
Rat IgM u chain
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgM and Rat Serum.  No reaction was observed against Rat IgG or Rat IgA.   Specificity was confirmed by ELISA at less than 5% cross reactivity against other Rat heavy or light chain isotypes.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Reactivity Notes

Reacts with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 794, Excitation Wavelength: 770, Label Molecular Weight: 1,050, Restore with 0.1 mL of deionized water (or equivalent).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Goat anti-Rat IgM Secondary Antibody [DyLight 800]

  • AGM1
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • IgM
  • immunoglobulin heavy constant mu
  • MGC104996
  • MGC52291
  • MU
  • VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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