Recombinant Human IGHG1 Protein

SDS-Page: Recombinant Human IGHG1 Protein [NBP3-18523] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Bioactivity: Recombinant Human IGHG1 Protein [NBP3-18523] - Recombinant Human Fc gamma RIIIA/CD16a His Protein (NBP2-53046) when coated at 10 ug/ml (100 ul/well) can bind Human IgG1 Fc. The ED50 range is less than or equal to 1.5 ug/ml.

Human Fc gamma RIIIA/CD16a is coated at 10 ug/ml (100 ul/well) can bind NBP3-18523. The ED50 range is less than or equal to 4 ug/ml.

Recombinant Human IgG1 Fc when coated at 1ug/ml (100 ul/well) can bind Human Fc gamma RIIA/CD32a in a Functional ELISA assay.

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Concentration: 1 mg/ml

Recombinant Human IGHG1 Protein Summary

• Description: A recombinant protein corresponding to amino acids 100-330 of Human IGHG1
  
  

  Source: HEK293

  Accession Number: CAC20454.1

  Uniprot ID: P01857

  Amino Acid Sequence: PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K

• Details of Functionality: Measured by its binding ability in a functional ELISA with Recombinant Human Fc gamma RIIIA/CD16a His Protein. The ED50 range is less than or equal to 4 ug/ml.
• Source: HEK293
• Protein/Peptide Type: Recombinant Protein
• Gene: IGHG1
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 25.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human IGHG1 Protein

• immunoglobulin heavy constant gamma 1 (G1m marker)

Background

IGHG1( AAH06402, 1 a.a. - 480 a.a.) full-length recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IGHG1