Mouse IgG2b Kappa Light Chain Isotype Control (MG2b-57)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB120-18421
    • Availability
      Product Discontinued

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Mouse IgG2b Kappa Light Chain Isotype Control (MG2b-57) Summary

Description
The MG2b-57 immunoglobulin is Mouse IgG2b Kappa isotype and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed Mouse, Rat and Human tissues.
Immunogen
TNP and KLH
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
ICC/IF, IHC-Paraffin, IP and WB usages were reported in scientific literature. Purity is greater than 95% by SDS-PAGE. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.
Publications
Read Publication using NB120-18421.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
0.2% BSA, PBS, pH 7.2.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Notes

Endotoxin level < 0.01 ng/ug.

Background

MG2b-57 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2b Kappa Light Chain Isotype Control (NB120-18421)(1)

We have publications tested in 1 application: FLOW.


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Publication using NB120-18421 Applications Species
Magatti M et al. Stem Cells 26:182. 2008 [PMID: 17901399] (FLOW) FLOW

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Additional IgG2b Kappa Light Chain Products

IgG2b Kappa Light Chain NB120-18421

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