Rat IgG2a Kappa Isotype Control

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF
Host
Rat
Conjugate
Unconjugated

Order Details

Rat IgG2a Kappa Isotype Control Summary

Description
This Rat IgG2a Kappa immunoglobulin is useful as an isotype control for Rat IgG2a Kappa antibody.
Specificity
In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1a, IgG1b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
Isotype
IgG2a Kappa
Host
Rat
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
Application Notes
Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20 degrees C or below. Avoid cycles of freezing and thawing. RAT IgG2a Kappa (?) isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: None.
Preservative
0.01% Sodium Azide
Purity
Protein G purified

Background

RAT IgG2a Kappa (?) isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain a - IgA, ? - IgG 1, 2a, 2b, 2c and u - IgM, light chain ? and ?.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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IgG2a Kappa NBP2-31381

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