Mouse IgG2a Isotype Control

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF
Host
Mouse
Conjugate
Unconjugated

Order Details

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Mouse IgG2a Isotype Control Summary

Description
This Mouse IgG2A immunoglobulin is useful as an isotype control for Mouse IgG2A antibody.
Isotype
IgG2a
Host
Mouse
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
Publications
Read Publication using NBP1-96778.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Stabilizer: None.
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for Mouse IgG2a Isotype Control

  • IgG2A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2a Isotype Control (NBP1-96778)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG2a Isotype Control (NBP1-96778). (Showing 1 - 2 of 2 FAQ).

  1. I would like to know the clonality of this sample. Is this monoclonal or polyclonal?
    • Their is no clonality for this product as it is an isotype control, and contains multiple different non-specific IgG2a immunoglobulins that are non-specific, to rule out binding with Fc receptors in flow and other experiments requiring this type of control.
  2. I want to know how the Ab was purified. More specifically, if it was with chomatography (like anti-mouse IgG columns, or other) OR with protein A or protein G columns. The latter is important to us to take presence of trace amounts of protein A or protein G into consideration.
    • This sample is a mouse IgG2a Isotype Control, which has been prepared from immunodeficient mouse ascites by protein A chromatography using specific conditions for subclass purification. Typically less than 1% cross reactivity against other mouse and human heavy or light chains isotypes was detected by ELISA.

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