Rat IgG1 Isotype Control Summary
This Rat IgG1 immunoglobulin is useful as an isotype control for Rat IgG1 antibody.
In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG2a, IgG2b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
Protein G purified
- Western Blot 1:100 - 1:2000
- ELISA 1:100 - 1:2000
- Flow Cytometry 1:1000 - 1:5000
- Fluorophore-linked immunosorbent assay 1:100 - 1:2000
- Immunocytochemistry/Immunofluorescence 1:10 - 1:500
- Immunohistochemistry 1:10 - 1:500
- Immunohistochemistry-Paraffin 1:10 - 1:500
Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20 degrees C or below.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: None.
0.01% Sodium Azide
Protein G purified
Alternate Names for Rat IgG1 Isotype Control
RAT IgG1 Lambda (?) isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain a - IgA, ? - IgG 1, 2a, 2b, 2c and u - IgM, light chain ? and ?.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed
for 1 year from date of receipt.
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