Mouse IgG1 Isotype Control

Images

 
SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Mouse IgG1 Isotype Control Summary

Description
This Mouse IgG1 immunoglobulin is useful as an isotype control for Mouse IgG1 antibody.
Specificity
NBP1-97005 is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
Application Notes
Mouse IgG1 control has no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-97005 in the following applications:

Publications
Read Publication using NBP1-97005.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: None.
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for Mouse IgG1 Isotype Control

  • IgG1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG1 Isotype Control (NBP1-97005)(1)

We have publications tested in 1 application: WB.


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Review for IgG1 Isotype Control (NBP1-97005) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NBP1-97005:
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Flow
(1)
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Human
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Images Ratings Applications Species Date Details
Flow Cytometry IgG1 NBP1-97005
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reviewed by:
Anonymous
Flow Human 04/21/2015
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Summary

ApplicationFlow Cytometry
Sample Testedhuman fetal derived neural stem cells
SpeciesHuman
Lot28929

Blocking

Blocking DetailsN/A

Primary Anitbody

Dilution Ratiomixed in a 1:50 dilution of 1XPBS for 30 minutes at room temperature

Secondary Antibody

Secondary Descriptiongoat anti-mouse APC/Cy7
Secondary Manufacturer Cat#Abcam, ab130785
Secondary Concentration1:50

Details

Detection Notesdetected on flow cytometry, RL2 (750nm)
Fixation Detailssample fixed in PFA for 15 minutes at room temperature
Permeabilization DetailsN/A
Wash Descriptionsamples were washed with 1XPBS at room temperatue, 3mL per wash

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG1 Isotype Control (NBP1-97005). (Showing 1 - 1 of 1 FAQ).

  1. I would like to know the host strain of this product?
    • This isotype control was purified from ascites and consists of antibody from Balb/c with possible trace amounts from Swiss webster.

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Blogs on IgG1

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Recent Reviews

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Anonymous
04/21/2015
Application: Flow
Species: Human

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